Regenerative capacity of mammals is limited and can rarely regenerate a specific organ or tissue fully. Due to these limitations, regenerative medicine seeks efficient and safe cell sources for regeneration of damaged tissues and organs or treatment for incurable diseases. Human embryonic stem cells (HESCs) hold two important properties called self renewal and pluripotency. However, the use of ...

regenerative capacity of mammals is limited and can rarely regenerate a specific organ or tissue fully. due to these limitations, regenerative medicine seeks efficient and safe cell sources for regeneration of damaged tissues and organs or treatment for incurable diseases. human embryonic stem cells (hescs) hold two important properties called self renewal and pluripotency. however, the use of ...

The extract of pluripotent stem cells induces dedifferentiation of somatic cells with restricted plasticity. In this study, we used the extract of human embryonic stem cells (hESC) to dedifferentiate adipose tissue-derived stem cells (ADSCs) and examined the impact of this reprogramming event on dopaminergic differentiation of the cells. For this purpose, cytoplasmic extract of ESCs was prepare...

s:3115:"Background: During reproductive life, the human endometrium undergoes around 500 cycles of growth, breakdown and regeneration. This outstanding regenerative capability is the basis for cyclic endometrial preparation and its dysfunction is involved in pathological disorders. Endometrial regeneration is mediated by the existence of a specialized endometrial stem cell (SC) population recen...

Studies on cell fusion-mediated nuclear reprogramming have led to the breakthrough of the induced pluripotent stem (iPS) cell technology. While this technology has neared stem cells to applications more than any other method, the mechanistic bases of reprogramming remain largely unsolved. In this context, comparative studies of oocyte and cell fusion-mediated reprogramming hold the greatest pro...

Background: At the outset of the somatic cell nuclear transfer (SCNT) process, the chromatin structure of the somatic cell which governs its state of differentiation undergoes dramatic changes, called reprogramming, and is compelled back to the embryonic stage. However, the overall epigenetic makeup of the resultant cloned embryos has been acknowledged far different from the fertilized embryos....

Background Despite successful cloning of many mammalian species, the cloning efficiency is extremely low compared to that of IVF raising the possibility of the existence of epigenetic barrier preventing successful cloning. MaterialsAndMethods Using comparative transcriptome analysis comparing transcriptomes of IVF and SCNT embryos and that of donor cells, we identified epigenetic barrier and fi...