To optimize Buruli ulcer (BU) microscopic diagnosis, we compared two smear preparation methods from tissue specimens: smears made with tissue suspension after grinding and smears made directly with unground tissue. We also compared two smear staining methods: auramine and Ziehl-Neelsen (ZN). IS 2404-PCR was used as reference method. One hundred and thirty-one surgical tissue specimens from pati...

Buffy coat smears from febrile patients may contain visible bacteria and therefore detect bacteraemia before conventional blood cultures become positive. However, it is unusual to see micro-organisms in an otherwise untreated peripheral blood smear. The case of a febrile neutropenic patient is reported. A Wright's stained peripheral blood smear contained bacterial elements, thus making earlier ...

Sepsis remains relevant especially among people with immunodeficiency. The article presents a case of sepsis rapid fatal outcome in an immunodeficient patient. diagnosis was promptly confirmed by detecting bacteremia microscopy stained smear the patient’s peripheral blood. publication discusses problems lifetime based on results general blood test. data need for correct assessment 5-diff hemato...

Group A beta-haemolytic streptococci were isolated from 51 (9.8%) of 519 patients with pharyngitis. Throat culture results showed the mean sensitivity, specificity and predictive value of a positive Gram-stained smear of pharyngeal secretions as 70%, 89% and 69%. The data suggest that Gram staining of a smear directly from the swab is as accurate as and more speedy than a culture from the phary...

A microscopic examination of an appropriately prepared and well-stained blood smear by a knowledgeable laboratory professional is necessary and clinically useful in a number of circumstances and for a variety of reasons. In this article, an attempt is made to delineate the purpose and criteria for blood smear examination in a variety of circumstances that are encountered in everyday laboratory ...

Leishmania tropica (cutaneous leishmaniasis) can be detected easily, rapidly, and conveniently by the examination of a skin ulcer smear that is stained with a modified method of Wright staining of blood (ie that used for routine hematological examinations).

A study was carried out in order to compare the detection of Trichomonas gallinae by examination smears throat swab and culture protozoa collected from both pigeon chicken. In present study, five media viz. Modified Diamond’s Media, Nutrient broth, Medium 199, Minimum Essential (MEM) Roswell Park Memorial Institute-1640 (RPMI-1640) were used for T. gallinae. Microscopic wet mount/ Giemsa staine...