Microbiological contamination of process waters is a current issue for pharmaceutical industries. Traditional methods require several days to obtain results; therefore, rapid microbiological methods are widely requested to shorten time-to-result. Milliflex Quantum was developed for the rapid detection and enumeration of microorganisms in filterable samples. It combines membrane filtration to un...

Membrane filtration is widely used for sterile filtration, flowthrough immuno-filtration, virus removal, and microbiological analysis.1,2,3,4,5,6 Prior work has described membranes that retain one protein over others.7,8 The purpose of this work is to capture a target species of living cells from a mixed population of microorganisms being concentrated in the retentate of solution being pushed t...

Abstract Filter membranes (pore size 0.2 μm) are frequently used to perform a sterile filtration. To characterize the pore of filter membranes, method determine particle retention nanoparticles was defined. The Dynamic Light Scattering first analysed and calibrated correlate concentration with absolute intensity scattered light. Afterwards, standard suspensions sizes 0.150 μm 0.239 were lead th...

The term 'ultrapure water' is a common way to define water used for on-line treatments: it refers to the absence of chemical, organic and microbiological contamination. To be more accurate in definition, every known and potential contaminant has to be fixed at its limit value. AAMI recommendations and various Pharmacopoeias have set limits for dialysate based on traditional dialysis treatments,...

The effect of different membrane filters on the bioactivity of tomato root exudates was tested in an in vitro assay addressing the germination of microconidia of the soil-borne fungus Fusarium oxysporum f. sp. lycopersici. Membrane filtration of unsterile root exudates with filters of different membrane materials and filter brands resulted in an increased microconidia germination. This effect v...

  White Spot Virus (WSV) is currently the most serious viral pathogen of shrimp worldwide it causes mortality up to 100% within 7-10 days in commercial shrimp farms. Infected Indian white shrimp Fenneropenaeus indicus samples were collected from Guatr shrimp site in Sistan and Baluchestan province in south of Iran and WSV infection was confirmed by Nested PCR. WSV was isolated from infected shr...

In the production of biopharmaceuticals disk-stack centrifugation is widely used as a harvest step for the removal of cells and cellular debris. Depth filters followed by sterile filters are often then employed to remove residual solids remaining in the centrate. Process development of centrifugation is usually conducted at pilot-scale so as to mimic the commercial scale equipment but this meth...