MICs for clinical Candida and Cryptococcus isolates were determined by a method incorporating the colorimetric indicator 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl] -2H-tetrazolium hydroxide (XTT), and the results were compared with MICs obtained by the National Committee for Clinical Laboratory Standards approved standard method (M27-A). One hundred percent of all isolat...

Susceptibilities of Madurella mycetomatis against amphotericin B and itraconazole in vitro were determined by protocols based on NCCLS guidelines (visual reading) and a 2,3-bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide (XTT) assay for fungal viability. The XTT assay was reproducible and sensitive for both antifungals. Itraconazole (MIC at which 50% of...

Objective
 The aim of this study was to compare four commonly
 used cytotoxicity assays (XTT, neutral red uptake, crys-
 tal violet assay, and propidium iodide staining) which
 analyzed the antiproliferative effects vitamin K2.
 Material Method
 Saos-2 cells, an osteosarcoma cell line, were
 exposed K2 at different concentrations (10
 μM, 20 30 40 50 100 ...

TOOL PRESENTATION: The paper introduces the HeKatE design environment called HaDEs. The HeKatE project aims at delivering new knowledge representation methods for rule-based systems. Principal ideas include an integrated hierarchical design process covering stages from conceptual, through logical to physical design. These stages are supported by speci c knowledge representation methods: ARD+, X...

The paper is dedicated to presentation of a new approach to joint design and verification of rule-based systems. The principal idea is that verification should be performed on-line, incrementally, during system design. This allows for early detection and handling of knowledge base anomalies and inconsistencies. The proposed approach offers also an innovative visual tool for computer-aided desig...

In recent years, there has been increased interest in the use of nanostructures various industries, such as food, textile, pharmaceutical, electronics, and chemical industries. Most these applications require a proper preparation specific nanomaterials. this study, we characterized silver nanoparticles (AgNPs) stabilized with polyvinylpyrrolidone prepared aqueous suspensions using dynamic light...

The increase in cellular and mitochondrial glutathione disulfide (GSSG) levels and the GSSG:GSH ratio after acetaminophen (AAP) overdose suggest the involvement of an oxidant stress in the pathophysiology. However, the initial severe depletion of hepatocellular glutathione makes quantitative assessment of the oxidant stress difficult. Therefore, we tested the hypothesis that oxidant stress prec...