BACKGROUND Potato virus Y (PVY, genus Potyvirus) causes substantial economic losses in solanaceous plants. Routine screening for PVY is an essential part of seed potato certification, and serological assays are often used. The commercial, commonly used monoclonal antibodies, MAb1128, MAb1129, and MAb1130, recognize the viral coat protein (CP) of PVY and distinguish PVYN strains from PVYO and PV...

Surface plasmon resonance (SPR)-based biosensors have been widely utilized for measuring interactions of a variety of molecules. Fewer examples include higher biological entities such as bacteria and viruses, and even fewer deal with plant viruses. Here, we describe the optimization of an SPR sensor chip for evaluation of the interaction of the economically relevant filamentous Potato virus Y (...

The photosynthetic rate of virus-infected plants is always reduced. However, the molecular mechanism underlying this phenomenon remains unclear. The helper component-proteinase (HC-Pro) of Potato virus Y (PVY) was found in the chloroplasts of PVY-infected tobacco, indicating some new function of HC-Pro in the chloroplasts. We generated HC-Pro transgenic plants with a transit peptide to target t...

In recent years, Potato Virus Y has reemerged as a serious disease problem in many potato production areas in the northern United States and eastern Canada. Three recently introduced cultivars, ‘Russet Norkotah’, ‘Shepody’, and ‘Silverton’ are widely grown and express mild or no symptoms when infected with PVY. The lack of symptoms often associated with these cultivars and novel, recombinant st...

A major justification for taxonomic research is its assumed ability to predict the presence of traits in a group for which the trait has been observed in a representative subset of the group. Similarly, populations in similar environments are expected to be more alike than populations in divergent environments. Consequently, it is logical to assume that taxonomic relationships and biogeographic...

Potato virus Y (PVY) the type member of the genus Potyvirus, occurs world-wide as isolates which differ in host range and the type of symptoms caused. The sequences of a 5' segment of viral RNA overlapping the 5' non-translated region (5'NTR) alone (ten isolates) or the 5'NTR and the adjacent P1 coding region (eight isolates) were established. These data were used to quantify the polymorphism i...

Abstract Potato virus Y (PVY) is one of the main diseases tobacco (Nicotiana tabacum). Recent studies indicated that Va gene (Ntab0942120) determines susceptibility crop to PVY, and product interacts with PVY genome-linked protein (VPg) initiate genome translation process, which ultimately leads systemic infection by virus. In this research, cultivar LJ911 was used as receptor material for edit...

The potential effect of genetic modification on nutritional properties of potatoes transformed to improve resistance to a necrotic strain of Potato virus Y was determined in a rat experiment. Autoclaved tubers from four transgenic lines were included to a diet in the amount of 40% and compared with the conventional cv. Irga. The experiment lasted 3 weeks and special attention was paid to nutrit...

SIEVERT, R. C. 1978. Effect of potato virus Y and tobacco mosaic virus on field-grown burley tobacco. Phytopathology 68: 823-825. Yield, grade index, crop index, value per kilogram, and tobacco had less nicotine and TMV-infected tobacco had less value per hectare were reduced when Burley 37 became a-amino nitrogen, but tobacco infected with TMV+PVY had infected with tobacco mosaic virus (TMV), ...