in the present study, random amplified polymorphic dna (rapd) and inter-simple sequence repeat (issr) markers were used to estimate genetic diversity and relationships among 35 cluster bean [cyamopsis tetragonoloba (l.) taub] genotypes. for rapd analysis, 20 random primers were used which amplified 164 bands, 147 of which were polymorphic with an average polymorphism of 90.3%. the amplified pro...

Background: Species identification in commercially processed food and feed products is one of the important issues. This study was conducted to develop a genetic method for the detection of pig and cattle species in processed food and feed products using newly designed species-specific primers targeting mitochondrial 12S rRNA gene fragments. Methods: Two sets of specific primers were designed ...

the effect of sub culturing frequencies and different cytokinins (benzyl amino purine (bap), 2-isopentenyl adenine (2ip) on genetic stability of micropropagated shoots from olive plant were investigated by using physiological traits and random amplified polymorphic dna (rapd) markers. axillary buds of the olive (cv. dezful) plants were cultured and subcultured in dkw medium, supplemented with b...

Methylation-specific PCR (MSP) of the mouse p53 gene has not yet been reported. We searched the CpG islands, sequenced the bisulfited DNA, and designed PCR primers for methylation and unmethylation sites. DNA from a young mouse produced a strong PCR product with the unmethylated primer and a weaker band with the methylated primer. DNA from an old mouse produced bands of similar intensities with...

Eleven adenovirus strains including six ovine prototypes, three uncharacterized ovine and bovine isolates and two uncharacterized llama isolates were digested with four different restriction enzymes (RE). This investigation was conducted in order to identify their specific RE patterns which may help in comparing different isolates and assist in classification. Digested viral and cellular DNA sa...

Carvalho M., Matos M., Carnide V., 2014. Fingerprinting of Vaccinium corymbosum cultivars using DNA of fruits. Hort. Sci. (Prague), 41: 175–184. In recent years the production and consumption Vaccinium corymbosum has increased. Highbush blueberry cultivars are divided into three types, northern, intermediate and southern. The traditional methods for classification of highbush blueberry cultivar...

Studies of V79-171 cells were undertaken to determine what extracel lular or intracellular derivative of the drug WR-2721 is associated with radioprotection. The effect of preincubation at 23 ±1°Cwith WR-2721, and with derivatives of WR-2721 produced in medium containing alkaline phosphatase, upon survival of cells following subsequent -y-irradiation was examined. It was established that WR-2...