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The human interferon fi (IFN-$) regulatory element consists of multiple enhanson domains which are targets for transcription factors involved in inducible expression of the promoter. To further characterize the protein-DNA interactions mediating IFN-$ induction, positive regulatory domain (PRD) II binding proteins were purified from phorbol ester induced Jurkat 1-cells and from IFN primed, cycl...
We have investigated the effects of phorbol ester 12-0-tetradecanoylphorbol-13-acetate (TPA) and permeant diacylglycerol 1,2-dioctanoylsn-glycerol (l)if„)on MCF-7 cell proliferation and protein kinase C activity. l)i< Hmimics the effects of TPA on both cell morphology and proliferation, with an ED»value of 11 ^g/ml for cell growth inhibition. As with TPA and phorbol 12,13-dibutyrati-, DiC'ne...
AÃ--(5-|Ar-(3,4-dihydro-2-methyl-4-oxoquinazolin-6-ylmethyl)-/Vmethylamino]-2-thenoyl)-i.-glutamic acid (ICI D1694) is a water-soluble, folate-based thymidylate synthase (TS) inhibitor designed to be a less toxic and more potent analogue of the clinically tested /V'u-propargyl-5,8dideazafolic acid. Inhibition of isolated L1210 TS by ICI D1694 is mixed noncompetitive (although tending toward com...
A typical computer representation of a design includes geometric and physical information organized in a suitable combinatorial data structure. Queries and transformations of these design representations are used to formulate most algorithms in computational design, including analysis, optimization, evolution, generation, and synthesis. Formal properties, and in particular existence and validit...
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