An improved extraction and HPLC method for the simultaneous extraction and quantitation of retinol, α-tocopherol, α-tocotrienol and β-carotene was developed to analyze commercial whole/semi-skim/skim samples of raw/pasteurized/UHT milk in transparent plastic/glass bottles and Tetra Brik containers. The sample preparation method required prior saponification at 40oC for 15 min followed by n-hexa...

Xestospongia sp. merupakan salah satu sponge dari kelas Demospongiae yang digunakkan sebagai bahan baku obat dapat dikembangkan satunya dengan metode SNEDDS. Penelitian ini bertujuan untuk mengetahui formulasi dan karakteristik SNEDDS ekstrak etanol sp., Formulasi estrak menggunakan VCO fase minyak, Tween 80 surfaktan PEG 400 ko-surfaktan. Formula terbaik didapatkan adalah 8 komposisi yaitu 1 m...

The biochemical properties of invertase entrapped in alginate gel were studied. The kinetic parameters were determined for immobilized and free invertase. The value of Michaelis constant Km of the immobilized invertase (139.19 mM) was greater than that of the free invertase (93.19 mM), whereas, Vmax was smaller for the immobilized enzyme. Immobilization impressively improved the thermal and sto...

Bacteriocin producing Lactobacillus acidophilus and Pediococcus acidilactici were isolated from milk and meat samples, respectively. An attempt was made to produce bacteriocin in a Dairy Based (DB) medium using these organisms. Higher bacteriocin activity was shown by L. acidophilus in the DB medium. Bacteriocins of both the organisms were effective against food pathogens. The bacteriocins were...

Cryopreservation is the method of choice for long term storage of human PBMCs. This study was designed to compare the different combinations of variables affecting the cryopreservation of PBMCs samples. The viability of PBMCs separated from 2×5 ml peripheral blood samples obtained from 16 healthy adult volunteers, were measured using trypan blue dye exclusion method just before freezing with ...

cryopreservation is the method of choice for long term storage of human pbmcs. this study was designed to compare the different combinations of variables affecting the cryopreservation of pbmcs samples. the viability of pbmcs separated from 2×5 ml peripheral blood samples obtained from 16 healthy adult volunteers, were measured using trypan blue dye exclusion method just before freezing with di...

The changes in physicochemical properties of salt-soluble proteins and gel prepared with chicken breast muscles during −1.5oC (ice-temperature) 4oC (refrigerated temperature) storage were investigated. Compared the samples stored group, decrease solubility, content total sulfhydryl available groups SSP from at was effectively delayed, increase both surface hydrophobicity disulfide bond obviousl...