The identification of lysine acetylation of steroid hormone receptors has previously been based on the presence of consensus motif (K/R)XKK. This study reports the discovery by mass spectrometry of a novel progesterone receptor acetylation site at Lys-183 that is not in the consensus motif. In vivo acetylation and mutagenesis experiments revealed that Lys-183 is a primary site of progesterone r...

Post-translational modification of proteins by lysine acetylation plays important regulatory roles in living cells. The budding yeast Saccharomyces cerevisiae is a widely used unicellular eukaryotic model organism in biomedical research. S. cerevisiae contains several evolutionary conserved lysine acetyltransferases and deacetylases. However, only a few dozen acetylation sites in S. cerevisiae ...

Acetylation is a well-established process to improve dimensional properties of wood and resistance fungi attack. This study was carried out due limited studies on the effect acetylation mechanical properties, thermal stability, microstructural aspects wood. Wood blocks, (each 20×20×60 mm) Obeche were acetylated with acetic anhydride at 120 °C for 60, 120, 180, 240 300 minutes. The microstructur...

the acetylation of various alcohols and phenols was performed successfully using acetic anhydride in the presence of citric acid coated magnetite nanoparticles as catalyst under solvent-free condition and at 45 °c. the catalyst showed high thermal stability and was recovered and reused at least 5 times without any considerable loss of activity. the present process is environmentally benign and ...

To visualize histone acetylation in living cells, we developed a genetically encoded fluorescent resonance energy transfer (FRET)-based indicator. Response of the indicator reflects changes in the acetylation state of both K5 and K8 in histone H4. Using this acetylation indicator, we were able to monitor the dynamic fluctuation of histone H4 acetylation levels during mitosis, as well as acetyla...

this research was conducted to investigate the effect of acetylation and type of resin on fire resistance of one and three layered populus particleboard. populus particles were acetylated at three levels of acetyl content (wpg of 0, 8.39% and 17.27%). in manufacturing of one and three layered boards with acetylated particles in all thickness or surface of the boards two type of resin, urea form...

Protein acetylation affects synaptic plasticity and memory, but its effects on synapse composition have not been addressed. We found that protein acetylation promotes the dendritic clustering of the excitatory postsynaptic scaffold protein PSD95 in hippocampal neurons, without affecting the total levels of this protein. Cortactin, an F-actin-binding protein enriched in dendritic spines, is a su...

Tetrahydropyranylation of alcohols and phenols, acetylation of aldehydes and N-Boc protection of amines are efficiently catalyzed by melamine trisulfonic acid (MTSA). All reactions were performed at room temperature in good to high yields.

The HMG-CoA reductase inhibitor simvastatin activates AMP-activated protein kinase (AMPK) and thereby induces histone acetylation. We postulated that combining with the deacetylase (HDAC) romidepsin would kill bladder cancer cells by inducing acetylation cooperatively. combination of induced robust apoptosis killed synergistically. In murine subcutaneous tumor models using MBT-2 cells, a 15-day...