Heparin binding proteins (HBPs) are produced by accessory glands. These are secreted into the seminal fluid, bind to the spermatozoa at the time of ejaculation, favour capacitation, acrosome reaction, and alter the immune system response toward the sperm. The present study was conducted with an objective to assess the effect of purified seminal plasma-HBPs (SP-HBPs) on cross bred cattle bull sp...

The aim of this study was to evaluate sperm quality following separation and selection using a silane-coated silica colloid method (PureSperm ® ) or Percoll ® . Frozen/thawed sperm from buffalo bulls (n=5) were assigned to two groups. The first group was separated using a Percoll ® discontinuous gradient (45 and 90%) and the second group by a single layer of PureSperm ® . Sperm motility, concen...

Frozen-thawed semen from six Boer bucks was pooled and used to compare the recently available commercial product CapriPure with Percoll for sperm quality. Sperm quality parameters such as sperm motility, concentration, membrane integrity (PI/CFDA), acrosome integrity (FITC-PNA) and mitochondrial activity (JC-1) were evaluated before and after sperm processing using CapriPure and Percoll density...

AIM The present study was carried out on semen ejaculates of 15 Karan Fries (KF) bulls maintained at Artificial Breeding Research Centre, National Dairy Research Institute, Karnal, India with an objective to evaluate the relationship between the conventional and fluorescent based semen quality analysis of the bulls. MATERIALS AND METHODS A total of 96 ejaculates were collected from 15 KF (Hol...

the aim of this study was to investigate the effect of adding different levels of zinc sulfate in semen extender on bovine sperm quality during cryopreservation. semen was collected from 4 holstein bull twice a week and the proper samples were pooled. semen were allocated to four extender treatments consisted of 0, 50, 100, 150 µm of zinc sulfate in 5 replicates and then, were frozen. post-thaw...

In order to improve Boer goat semen quality during cryopreservation process, the influence of sugar supplementation on semen characteristics of sperm were investigated. Three experiments were carried out to investigate the effect of (a) addition of two monosaccharides (fructose and glucose) and two disaccharides sugars (trehalose and sucrose) (b) sugar combination (fructose and trehalose, sucro...

Introduction: Oxidative stress (OS) is an important factor in the etiology of male infertility which presents abnormal increase in the production of free radicals with negative influences on reproduction. The aim of present study is evaluation effect of mercury (Hg) (600& 1200 micro molar) and nicotine (0.75 mM), in vitro, on membrane integrity (LPO), GSH content, motility, and acrosome reactio...

background: the glycoconjugate content of sperms indicates their physiological and fertility properties. lectin reactivity is indicative of intact, capacitated, and acrosome-reacted sperms. in the epididymis, sperms experience maturation, glycoconjugate modification, and simultaneously, higher l-carnitine (lc) concentrations. the aim of this project was to evaluate the effects of lc and pentoxi...

Background Low-density lipoproteins (LDL) is known to protect boar sperm during freezing–thawing, but little information is known about the effects of LDL extracted from different avian egg yolks on post-thaw boar semen quality. The purpose of this study was to compare and analyze the effects of LDL at various concentrations and different species on boar sperm quality after freezing–thawing. Ma...

the goal of this study was the evaluation of semen extender filtration on post-thawed buck sperm quality. a total 16 ejaculates were collected from four buck (4 ejaculates from each buck) twice a week. after semen collection and primary evaluation, collected semen samples were pooled and divided into two equal parts. each part was diluted with one of the following extenders: 1) non-filtered lec...