The study included the extraction and purification of glucose oxidase enzyme from Penicillium chrysogenum, pure by precipitation with ammonium sulfate at a saturation rate 70% specific activity in this step reached (10.802) units/mg fold 1.276, was followed dialysis step, 13,593 48.184. Finally, ion exchange chromatography using DEAE-Cellulose column, 19.355 units/mg, 36.59, after which migrate...

Ionic liquids are a very important class of compounds due to their remarkable properties and wide range applications. On the other hand, capillary electrophoresis is also gaining importance in separation science because its fast speed inexpensive nature. The use ionic continuously. present review article describes applications electrophoresis. This general aspects electrophoresis, optimization ...

Permanent protein-protein interactions are commonly identified by co-purification of two or more protein components using techniques like co-immunoprecipitation, tandem affinity purification and native electrophoresis. Here we focus on blue-native electrophoresis, clear-native electrophoresis, high-resolution clear-native electrophoresis and associated techniques to identify stable membrane pro...

The challenge associated with the use of synovial fluid for osteoarthritic proteome studies is optimization step, which involves depletion high abundant proteins from samples. objective this study was to develop a cost efficient and effective method remove albumin canine studies. Pooled samples were obtained clinically healthy dogs (n=5), no radiographic features osteoarthritis. acetone precipi...

Medicinal plants have been used in traditional medicine for a long time. These contain phytochemicals that variety of medicinal properties. However, accurate identification and authentication plant species ensured their safety efficacy. DNA barcoding using molecular markers has proven to be useful method identification. The rbcL marker was detailed characterization, amplification, phylogenetic ...

The discovery that antigen}antibody interaction could be produced not only in liquids, but also on gel media, such as agar or agarose gels, with the formation of insoluble immunoprecipitates, opened the door to the development of the gel diffusion techniques for immunoprecipitation analysis. The Rrst of these techniques, known as double diffusion, was introduced by Ouchterlony in 1948. In this ...

Image analysis is an image processing technique that aims to extract features or information from images. Image analysis in medicine has a special place because is a basis for disease diagnosis for physicians. Electrophoresis is a laboratory separating technique. Electrophoresis images are created during the electrophoresis process. Serum protein and hemoglobin electrophoresis test are the ...

Methylsensitive comet electrophoresis is based on the assessment of level DNA migration from individual lysed cells after treatment with methylsensitive restriction enzymes. Using model human lymphocytes, optimal combination intensity and time was selected a new approach for evaluating relative methylation proposed. It established that in T98G culture, which are actively proliferating, higher t...