Human amniotic membrane has been used as a material to accelerate wound healing and reconstruct damaged organs. The aim of the present study was to assess histologically human amniotic membrane transplantation on rabbit's gingival wound. Three- to 4-month-old male rabbits were divided into 2 groups, i.e., control (group I) and amniotic membrane-transplanted animals (group II). Buccal gingival w...

PURPOSE To introduce autologous tragal perichondrium transplantation as a novel surgical modality for the management of intractable symptomatic bullous keratopathy. METHODS In three eyes of three patients with painful bullous keratopathy, autologous tragal perichondria were transplanted on the corneal surface with the human amniotic membrane transplanted above. We included an additional three...

The purpose of this study is to analyze clinical experience about the effects of human amniotic membrane transplantation in eyes with neurotrophic ulcers. In 11 eyes the application of amniotic membrane was performed since January 1999 because of neurotrophic ulcers. The follow up period was longer than 12 months: 19.7+/-6.0 months. The average healing period after the surgery was 1.6+/-0.6 wee...

PURPOSE To clarify the usefulness of multilayer amniotic membrane transplantation in an unusual case of calcareous corneal degeneration in a patient with graft-versus-host disease. METHODS A 20-year-old Caucasian woman had bilateral calcareous corneal degeneration of one year of evolution, secondary to graft-versus-host disease. Treatment for both eyes with topical steroids and antibiotic oin...

Human amniotic membrane is a standard substratum used to culture limbal epithelial stem cells for transplantation to patients with limbal stem cell deficiency. Various methods were developed to decellularize amniotic membrane, because denuded membrane is poorly immunogenic and better supports repopulation by dissociated limbal epithelial cells. Amniotic membrane denuding usually involves treatm...

CASE REPORT A 74-year-old man developed a partial limbal stem cell deficiency secondary to a chemical injury in the left eye. He was treated with a sectorial conjunctival epitheliectomy and amniotic membrane transplantation. After 3 weeks there was an intact, smooth and stable corneal epithelium. DISCUSSION This case report emphasizes that conjunctival epitheliectomy and amniotic membrane tra...

Purpose: To report a case with corneal ulcer after keratoplasty connected to long-term use of topical 0.1% diclofenac sodium. Methods: A 81-year-old man presented with corneal ulceration. The patient’s clinical history showed that he had keratoplasy after ocular trauma for about 10 years ago. He was using topical dexamethasone sodium phosphate 0.1% and topical diclofenac sodium 0.1% for one yea...

The purpose of this study is to analyze the clinical experience and the effect of human amniotic membrane transplantation on pterygium excision and bullous keratopathy. From January 1999 to January 2001 at University Hospital »Sestre milosrdnice« amniotic membrane transplantation was performed consecutively in 21 eyes: 11 eyes with bullous keratopathy and 10 with recurrent pterygia. In the grou...

purpose: to evaluate the role of amniotic membrane transplantation (amt) in acute ocular chemical burns. methods: patients with grade ii-iv (gii-giv) ocular chemical burns (roper-hall classification) of less than two weeks’ duration underwent amt in addition to medical therapy. patients were followed for at least 6 months and evaluated for ocular pain, visual acuity, epithelial defect healing, ...