We report for the first time the use of two photon fluorescence as detection method of affinity binding reactions. We use a resonant grating waveguide structure as platform enhancement for detecting the interaction between fluorescent labeled Boldenone, a non-natural androgenic hormone, and a specific anti-anabolic antibody. We were able to detect a surface coverage of approximately 0.7 ng/mm(2).

used in the production of plastic polycarbonate, have been shown to make disorders in sexual organs including the prostate gland [4]. Such environmental hormones may play a critical role in influencing for triggering prostate disease [5]. While numerous factors are involved in prostate carcinogenesis [6,7], epidemiological studies have reported a relationship between elevated circulating estrog...

Numerous experimental data support the idea that ovarian tissue in mammals secretes androgenic hormone in addition to the female sex hormones.2'4'14 Similar observations have been made on the starling,"5 the night heron,9 and the English sparrow.8' 15 In the case of the sparrow the evidence is based on the observation that the bill of the female darkens appreciably during the breeding season or...

It is generally agreed that estrogens, and possibly androgens, are important in the etiology of breast cancer, but no consensus exists as to the precise estrogenic or androgenic environment that characterizes risk, or the exogenous factors that influence the hormonal milieu. Nearly all the epidemiological studies conducted in the 1970s and 1980s were hospital-based case-control studies in which...

OBJECTIVE To examine levels of C reactive protein in users of anabolic androgenic steroids (AAS) compared with age matched control groups consisting of AAS using (but abstinent)/resistance trained and non-drug using/sedentary controls. METHOD Subjects included AAS using bodybuilders (n = 10); bodybuilders who denied AAS use (n = 10); sedentary controls (n = 8). Venous blood was sampled, from ...

Phytoecdysteroids are structural analogs of the insect molting hormone ecdysone. Plants comprise rich sources of ecdysteroids in high concentration and with broad structural diversity. Ecdysteroids have a number of proven beneficial effects on mammals but the hormonal effects of ecdysteroids have been proven only in arthropods. Their structures are somewhat similar to those of the vertebrate st...