This study was aimed to investigate the effects of omega-3, 6, 9 fatty acids on the characteristics of bovine chilled and frozen-thawed semen. For this purpose, oil containing different levels of omega-3, 6, 9 fatty acids were added to semen extender. To emulsify the oil in semen extender, polyethylene glycol (PEG) was added as a suitable solvent and the solution was finally sonicated. Five pro...

This study was aimed to investigate the effects of omega-3, 6, 9 fatty acids on the characteristics of bovine chilled and frozen-thawed semen. For this purpose, oil containing different levels of omega-3, 6, 9 fatty acids were added to semen extender. To emulsify the oil in semen extender, polyethylene glycol (PEG) was added as a suitable solvent and the solution was finally sonicated. Five pro...

The aim of this study was to determine the effect of low levels of glutathione on post-thawed buck sperm quality. In this experiment, different concentrations of glutathione [0 (LG-0), 0.5 (LG-0.5), 1 (LG-1), 1.5 (LG-1.5), and 2 (LG-2) mM] were added in a soybean lecithin-based extender. A total of 16 ejaculates from four bucks were collected and pooled. Each pooled sample was divided into five...

the effect of osmolarity and glycerol different levels in soybean lecithin-based extender on the bull sperm quality after cryopreservation was examined using six holstein bulls in a 2 × 3 factorial trial based on completely randomized design, with three levels of osmolarity (250, 300 and 350 mosml) and two levels of glycerol (5 and 7 %) .in general, semen samples were collected 36 times (from e...

Cryopreservation of caudal epididymal spermatozoa is an effective technique to conserve genetic potentials of superior dogs when it is not possible to collect ejaculated spermatozoa. Although hen egg yolk is commonly supplemented into the semen extender, active substances within the egg yolk which protect sperm against cryoinjury remain to be discovered. Among its compositions, low-density lipo...

The study was conducted to determine the influence of D-aspartic acid (D-Asp) as antioxidant supplement and thawing rates on ram sperm motility, membrane integrity, abnormality, viability, mitochondria activity, malondialdehyde and antioxidant activities, and total antioxidant capacity after freezing-thawing process. Semen samples from five mature rams (3-4 years old) were diluted with extender...

The study evaluated the effect of extenders and sperm concentration on migration efficiency Malabari buck spermatozoa in bovine cervical mucus. Fresh semen ejaculates collected six different days from three adult healthy bucks were pooled divided into four groups based extender used (tris-egg yolk-based or liposome-based extender) rate extension (200 400 million motile per 0.5 mL) (n=6 group) s...

This study was aimed to determine the effect of a freezing extender developed for Mesopotamian spiny eel (Mastacembelus mastacembelus Banks & Solander, 1794). Mesopotamian spiny eel was caught from May to July 2017 in the Atatürk Dam Lake and Euphrates Basin (meridians) in Şanliurfa, Turkey. During the study, the animals (n=10; 8 males, 2 females) were caught with gill nets (22 mm×22 mm, mesh s...

Background: Detection of sperm parameters based on conventional methods, immediately after thawing, have been revealed sperm capacity to in vivo and in vitro fertilization. But this evaluation is not fully reliable because of long journey of sperm during female genital tract to reach the oocyte and fertilize it. Therefore, analyzing postthawed sperm parameters after incubation is more beneficia...

the aim of this study was to determine the effects of the antioxidants superoxide dismutase (sod),glutathione peroxidase (gpx) and butylated hydroxytoluene (bht) on microscopic semen parameters andlipid peroxidation following the freeze-thawing of bull semen. ejaculates were collected from five holsteinbulls and pooled at 37°c. the semen samples were diluted with a cey extender containing addit...