Although Bicinchoninic acid (BCA) has been widely used to determine protein concentration, the mechanism of interaction between protein, copper ion and BCA in this assay is still not well known. Using the Micro BCA protein assay kit (Pierce Company), we measured the absorbance at 562 nm of BSA solutions with different concentrations of protein, and also varied the BCA concentration. When the co...

2’-Benzoyloxycinnamaldehyde (BCA) is a promising anticancer candidate against several types of cancers, including breast cancer. DJ-1 has been found to act as an oncogene, protecting cells against oxidative stress. A previous study showed that BCA-inducing antiproliferation was associated with continuous decrease in DJ-1 expression in MDA-MB-435 breast cancer cells. In this study, we found that...

Attenuation of estrogen receptor α (ERα) expression via unknown mechanism(s) is a hallmark of endocrine-resistant breast cancer (BCa) progression. Here, we report that miR-1271 was significantly down-regulated in letrozole-resistant BCa tissues and in letrozole-resistant BCa cells. miR-1271 directly targeted the chromatin of DNA damage-inducible transcript 3 (DDIT3) gene. miR-1271 expression le...

The debate that has raged in the legal literature for the last decade about benefit-cost analysis (BCA) in regulatory decision making about the environment has not been very productive for two key reasons: (1) it has focused on the way that BCA is used in the OIRA review process to “fine tune” 1 regulations just before they are issued, and (2) it has suffered from “selective realism” 2 by discu...

Tanshinone IIA (Tan-IIA) is an extract from the widely used traditional Chinese medicine (TCM) Danshen (Salvia miltiorrhiza), and has been found to attenuate the proliferation of bladder cancer (BCa) cells (The IC50 were: 5637, 2.6 μg/mL; BFTC, 2 μg/mL; T24, 2.7 μg/mL, respectively.). However, the mechanism of the effect of Tan-IIA on migration inhibition of BCa cells remains unclear. This stud...

Background and Aims: Small dense  low-density lipoproteins (sd-LDL) particles are smaller and heavier than typical LDL ones. They can penetrate into the endothelium of coronary arteries more easily because of their small size. Diabetes mellitus is accompanied by dyslipidemia such as increasing concentration of plasma very low density lipoprotein and sd-LDL. Peroxisome proliferator activated rec...

INTRODUCTION Bladder cancer (BCa) is among the most frequent cancer entities and relevantly contributes to cancer-associated deaths worldwide. The multi-protein Mediator complex is a central regulator of the transcriptional machinery of protein-coding genes and has been described to be altered in several malignancies. MED1, a subunit of the tail module, was described to negatively modulate expr...

With advances in local and systemic treatments, recurrence rates and risk of death secondary to breast cancer (bca) have been in continuous decline1. Breast cancer is among the malignancies most commonly encountered during pregnancy2,3: 0.2%–3.8% of cases are diagnosed during pregnancy and lactation4. The frequency increases with younger patient age: among women less than 30 years of age, 10%–2...

PURPOSE Our previous high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry study identified bladder cancer (BCA)-specific urine metabolites, including carnitine, acylcarnitines, and melatonin. The objective of the current study was to determine which metabolic pathways are perturbed in BCA, based on our previously identified urinary metabolome. MATERIALS AND METHO...

Primary central nervous system lymphoma (PCNSL) is a rare but often rapidly fatal form of non-Hodgkin B-cell lymphoma that arises within the central nervous system (CNS) and has a low propensity to metastasize. We performed immunohistochemistry on formalin-fixed, paraffin-embedded brain biopsy specimens from 24 patients with PCNSL to investigate the expression of B cell-attracting chemokine 1 (...