PURPOSE This study was performed to investigate the ability of recombinant human-bone morphogenic protein-2 immobilized on a heparin-grafted bone substrate to enhance the osteoblastic functions. MATERIALS AND METHODS The Bio-Oss®, not coated with any material, was used as a control group. In rhBMP-2-Bio-Oss® group, rhBMP-2 was coated with Bio-Oss® using only deep and dry methods (50 ng/mL, 24...

We compared results with three commercial folate radioassay kits [Bio-Rad, New England Nuclear (NEN), and RIA Products] with those by microbiological assay for more than 200 samples of human serum and whole blood. All but one kit (NEN) compared favorably with the microbiological assay for serum samples, although there were notable diagnostic discrepancies. Two kits (NEN and Bio-Rad) were tested...

Occasionally, a scientist makes a discovery which stands out clearly as the contribution which has had a profound effect on the subsequent developments and advance in a particular field of science. That Edgar Allen's introduction of the vaginal smear method' for detection of the "ovarian follicular hormone" was such a discovery is evidenced by the tremendous advances in our knowledge of ovarian...

Feature Article We develop approaches to realizing feedforward loops and certain memory functions (associative memory) with autonomous enzymatic-cascade biochemical processes. Such systems will offer possibilities for utilizing bio-inspired information handling steps on par with the presently used digital gates. Thus, we can be guided by nature's mechanisms in our experimenting with new informa...

Nanofiber scaffolds and bio-ceramic nanoparticles have been widely used in bone tissue engineering. The use of human induced pluripotent stem cells (hiPSCs) on this scaffold can be considered as a new approach in the differentiation of bone tissue. In the present study, a polyaniline-gelatin-polycaprolactone (PANi-GEL-PCL) composite nanoscaffold was made by electrospinning and modified superfic...

BACKGROUND The third-generation bio-intact parathyroid hormone (PTH) (1-84) assay was designed to overcome problems associated with the detection of C-terminal fragments by the second-generation intact PTH assay. The two assays have been compared primarily among dialysis populations. The present study evaluated the correlations and differences between these two PTH assays among patients with ch...

The bio-barcode amplification assay has become a powerful tool in detecting tens to hundreds of biological targets such as proteins and nucleic acids in the entire sample. However, current bio-barcode detection schemes still require many experimental steps including microarrayer-based immobilization of oligonucleotides on a glass chip, silver enhancement of immobilized gold nanoparticles on a c...

PURPOSE The aim of this study was to investigate in vitro cytotoxic activity of four methanolic crude plant extracts against panel cell lines. METHODS Methanolic extracts were tested for their possible antitumor activity and cytotoxicity using the 3-(4,5-dimetylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay on six cancer cell lines; non-Hodgkin's B-cell lymphoma (Raji), human leuk...