Selenium (Se) is an essential element for human health in trace amounts but harmful excess. Most plants contain a fairly low Se and crop supplements ensure adequate levels nutritional needs. Food the primary source of humans due to differences eating habits, its intake varies considerably. In body, selenium deficiency can lead diseases endocrine, musculoskeletal, cardiovascular, reproductive, n...

Introduction Angiogenesis plays a key role in the pathogenesis of numerous rheumatic diseases, such as rheumatoid arthritis, psoriatic arthritis, and vasculitides. Therefore, the inhibition of pathological angiogenesis may be considered a useful therapeutical approach in these rheumatic diseases. Methods This review article is based on a literature research about the role of biotechnological ...

Transient gene expression is one of the most common methods in molecular biology, equally relevant for basic research projects and biotechnological industries. Despite existence commercial transfection systems, which afford high efficiency levels reporter genes, expanding such systems to industrial scales often problematic due costs reagents. The well-described cationic calcium-phosphate are ac...

However, reproduction in sheep is seasonal, many breeds of sheep are able to mate not only in autumn, but out-of-season as well. The main factor determining seasonality is the photoperiod, but other factors can influence reproductive pattern, such as genetics, management practices and social cues. The fertility of spring and early summer breeding is usually lower; this imposes the need for alte...

Compared to other mammalian species, pig oocytes and embryos are characterized by high lipid contents stored mainly as lipid droplets in the cytoplasm. This fact has a negative influence on manipulations on oocytes and embryos and, in general, biotechnological procedures are much less advanced in pigs than in cows. This paper discusses current methods for modifying porcine oocytes and embryos u...

Microfluidics enables biotechnological processes to proceed on a scale (microns) at which physical processes such as osmotic movement, electrophoretic-motility and surface interactions become enhanced. At the microscale sample volumes and assay times are reduced, and procedural costs are lowered. The versatility of microfluidic devices allows interfacing with current methods and technologies. M...