Differential gene expression in sugarcane during sugarcane-Ustilago scitaminea interaction was conducted in a smut-resistant genotype. Using cDNA-AFLP along with silver staining, a total of 136 transcript-derived fragments (TDFs) were found to be differentially expressed in response to challenge by U. scitaminea. Forty TDFs, 34 newly induced plus six with obvious upregulated expression after in...

acquired azole resistance in opportunistic fungi causes severe clinical problems in immunosuppressed individuals. this study investigated the molecular mechanisms of azole resistance in clinical isolates of candida glabrata . six unmatched strains were obtained from an epidemiological survey of candidiasis in immunocompromised hosts that included azole and amphotericin b susceptible and azole r...

a member of homeodomain protein namely tgif2lx has been implicated as a tumor suppressor gene in human malignancy as well as in spermatogenesis. however, to our knowledge, dynamic functional evidence of the tgif2lx has not yet been provided. the aim of the present study was to investigate the human tgif2lx target gene(s) using a cdna-aflp as a differential display method. a pegfp-tgif2lx constr...

Sugarcane mosaic caused by Sugarcane Mosaic Virus (SCMV) is one of the most important virus diseases of sugarcane. In the present study, changes in the transcription profile obtained by cDNA-AFLP analysis were investigated in two sugarcane varieties contrasting to SCMV resistance, when challenged with a severe virus strain. Healthy plants derived from meristem tip tissue culture were mechanical...

On both recent and ancient time scales, polyploidy (genome doubling) has been a significant evolutionary force in plants. Here, we examined multiple individuals from reciprocally formed populations of Tragopogon miscellus, an allotetraploid that formed repeatedly within the last 80 years from the diploids T. dubius and T. pratensis. Using cDNA-AFLPs followed by genomic and cDNA cleaved amplifie...