نتایج جستجو برای: cdna aflpglioblastomahypoxia
تعداد نتایج: 41681 فیلتر نتایج به سال:
"اثرات یونی" و "اثرات اسمزی" دو جزء اصلی تنش شوری در گیاهان می باشند. شوری با ایجاد تنش یونی و تنش اسمزی موجب کاهش رشد و یا مرگ سلول می شود که در نهایت کاهش دهنده ی تولیدات زراعی است. در این تحقیق پاسخ مولکولی گیاه هالوفیت aeluropus littoralis تحت تیمار کلرید سدیم ( (nacl ، کلرید پتاسیم (kcl) و پلی اتیلن گلایکول peg)) در سه سطح 0(شاهد) و200 و 400 میلی مولار، یکسان سازی شده بر حسب پتانسیل اسمزی ...
یکی از گیرندههای کموکاینها در سطح نوتروفیلها il8rb است که برای حداکثر کارآیی نوتروفیلها در زمان بروز عفونت حیاتی است. تفاوت در توالی و بیان ژن il8rb منجر به ایجاد تفاوت در مقاومت به ورم پستان در جمعیتهای مختلف گاو میشود. هدف از این مطالعه بررسی تغییرات میزان بروز ژن il8rb در نتیجة آمیختهگری در گاوهای بومی استان گیلان با گاوهای اصیل هلشتاین است. به این منظور 56 نمونه خون از 5 جمعیت گاو ای...
background: the cultivation of saffron is expanding through the southeast of iran, and allergy to saffron pollen occurs in workers involved in processing this plant. we aimed to clone, sequence and express a major allergen involved in saffron pollen allergy, and to compare the recombinant with the natural allergen. methods: the n-terminal amino acid sequence of cro s 1, an allergen from saffron...
background: breast cancer is one of the most prevalent malignancies among women in various countries. her2 overexpression, which is due to different reasons, occurs in 20-30% of breast cancers. her2 gene encodes an 185kda transmembrane glycoprotein with 1255 amino acids. this active product triggers downstream intracellular signaling pathways inducing cell proliferation and cell survival. these...
Abiotic stresses, such as temperature (heat and cold), salinity, drought negatively affect plant productivity; hence, the molecular responses of abiotic stresses need to be investigated. Numerous genetic engineering studies have made substantial contributions revealed that are key factors associated with production losses in plants. In response altered expression patterns miRNAs been reported, ...
Aspartic proteases are a relatively small group of enzymes which express in various nematodes including Onchocerca volvulus. An estimation of the gene copy number corresponding to the OV7A clone, which contains a cDNA insert encoding approximately two-thirds of the entire coding sequence of aspartic protease of O. volvulus, was made by slot blot analysis in a closely related species O. gibsonig...
Adenylate kinases (ADK) are ubiquitous enzymes that contribute to the homeostasis of adeninenucleotides in living cells. In this study, the cloning of a cDNA encoding an adenylate kinase from the filariaOnchocerca volvulus has been described. Using PCR technique, a 281 bp cDNA fragment encoding part ofan adenylate kinase was isolated from an O. volvulus cDNA library. Use of this fragment as a p...
Purpose: The aim of this study was cloning the Gba enzyme in pUCBM21 plasmid, and making frame mutation on it and sequencing it. Materials and methods: mRNA was extracted from mouse spleen and glucocerebrosidase cDNA was synthesized and amplified by PCR with specific primers. cDNA was cloned in pUCBM21 and analyzed by restriction enzymes. A fragment of its sequence was deleted using MscI restr...
The role of RNA structure in virtually any biological process has become increasingly evident, especially the past decade. However, classical approaches to solving structure, such as crystallography or cryo-EM, have failed keep up with rapidly evolving field and need for high-throughput solutions. Mutational profiling sequencing using dimethyl sulfate (DMS) MaPseq is a sequencing-based approach...
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