Background and Aims: The aim of this study was cloning and expression of rabies virus glycoprotein by a eukaryotic expression plasmid pcDNA3.1(+) in BSR cell line. This construct might be used for a potential DNA vaccine. Materials and Methods: Glycoprotein gene was synthesized and cloned into pBluescript vector and then sub cloned into eukaryotic expression vector (pcDNA3.1(+)). After verifica...

abstract         background and objective: hirudin is an anticoagulant polypeptide secreted from the salivary glands of leeches. recombinant hirudin is a strong anticoagulant agent in arterial and venous thrombosis. the aim of this study was to evaluate the effect of inserting protein a signal peptide sequence of pezz18 plasmid on expression and secretion of the recombinant hirudin in e.coli.  ...

background and aims: newcastle disease virus (ndv) is one of the major pathogen in poultry. vaccination is intended to control the disease as an effective solution nevertheless this virus is a growing threat to the poultry industry. f gene open reading frame (orf) from ndv is 1650 bp, encoding a protein of 553 amino acids that can induce protective immunity alone. the f glycoprotein on the surf...

objective: in this project, our aim was to construct a novel expressing vector harboring a new sequence from overlapping region of ns3 gene of hcv from infected iranian patient. materials and methods: the partial ns3 (pns3) gene was amplified by nested-rt-pcr method using sera of hcv infected patients harboring genotype 1a. after purification and cloning the pns3 into ta-cloning vector, the be...

Background and Aims: DNA constructs containing HCV antigens have become one of the vaccine candidates for induction of anti-HCV cellular and humoral immunity. In this study, we constructed a novel expressing vector harboring a fusion sequence derived from an overlapping fragment in the middle of NS3 and a truncated core fragment to avoid troubles reported to be associated with full gene express...

human snake bitten poisoning is a serious threat in many tropical and subtropical countries such as iran. the best acceptable treatment of envenomated humans is antivenoms; however they have a series of economic and medical problems and need more improvements. in this study a combinatorial human immunoglobulin gene library against some of iranian snakes venoms was constructed. total rna prepare...

results cloning and subcloning of the flic gene were confirmed by colony pcr, restriction enzymes digestion and dna sequencing of the recombinant plasmids pprime-flic and pvax-flic. the expression of flagellin protein in eukaryotic cells was approved by immunofluorescence assay (ifa), western blotting analysis and the reverse transcriptase polymerase chain reaction (rt-pcr) method. conclusions t...

background: finding shows that there were several problems in the treatment of helicobacter pylori infection such as the emergence of resistance to the antibiotics, the risk of recrudescence, and the high cost of treatment. the ineffectiveness of conventional treatment mechanisms against cancer cells reveals the importance of peptides as a novel therapeutic approach. however, the short length o...

TA cloning is one of the simplest and most efficient methods for the cloning of PCR products. The procedure exploits the terminal transferase activity of certain thermophilic DNA polymerases, including Thermus aquaticus (Taq) polymerase. Taq polymerase has non-template dependent activity which preferentially adds a single adenosine to the 3'-ends of a double stranded DNA molecule, and thus most...