نتایج جستجو برای: cloning vector

تعداد نتایج: 249528  

A rapid DNA cloning system is a research interest of many scientists. TA cloning is one of the methods used for the cloning of PCR-amplified DNA molecules. The TA cloning method is a convenient and labor-saving replacement to traditional, restriction enzyme-mediated cloning strategies. A T-vector called pBlueskript ΙΙ SK-1 with the lethal gene ccdB was designed to construct a positive selection...

Journal: :avicenna journal of medical biotechnology 0

a highly efficient cloning vector was constructed for cloning pcr products by inserting an 80 bp dna fragment into pgem-5zf (+) vector. the xcm i digestion of this vector gave rise to a 3’ overhanging deoxythymidine offering the possibility of cloning pcr products with 3' adenosine overhang created by taq dna polymerase. furthermore, two ecor i sites were added to the construct for identificati...

Journal: :iranian journal of parasitology 0
b kazemi m bandehpour l maghen gh solgi

background: toxoplasma gondii is an obligate intracellular parasite and its sexual and asexual cycles, respectively take place in the intestinal epithelial cell of definitive host and tissue of intermediate hosts. congenital toxoplasmosis is more impor tant when the mother acquired the infection during pregnancy period for the first time. serological tests are the only meth ods for diagnosis of...

ابراهیمی, فیروز, حاجی زاده, عباس, رستمی, حسین, موسوی, سید جعفر,

Background and purpose: Clostridium botulinum bacteria produces seven types of botulinum neurotoxins among which types A, B, E and F are responsible for human botulism. One of the treatments for botulism is the inhibition of botulinum neurotoxins catalytic domain activity by inhibitors. In this study, botulinum neurotoxin type E catalytic domain has been cloned in pET28a vector and expressed in...

Ahmad Adeli, Fereidoun Mahboudi, Mohammad Azizi, Noushin Davoudi, Robert. W McMaster,

The pLE2SCX vector was developed for the stable expression of exogenous genes in the protozoan parasite Leishmania. The pLE2SCX construct contains three independent selection markers: herpes simplex virus thymidine kinase (HSV-TK), cytosine deaminase (CD) and streptothericin acetyltransferase gene (sat) in multiple cloning site, flanking by 5’ and 3’ untranslated regions of the previously clone...

ژورنال: پژوهش در پزشکی 2010
امامقلی پور1، , سلاله, بنده پور2، , مژگان, شعبانی1، , پریسا, کاظمی*, بهرام, سید2، , نگار , ماغن2، , لیلا , یغمایی1، , بهرام ,

Abstract Background: Factor VII, is a coagulant protease it begins the proteolytic cascade reactions and produces thrombin. The use of recombinant human factor VII, (rhFVII) is effective for the treatment of patients with hemophilia A or B. It is a target for gene therapy. This study was done to clone factor VII from HepG2 cell line. Methods: RNA was extracted from the hepatoma, (HepG2), ...

ژورنال: Medical Laboratory Journal 2013
Keyhanvar, N, Tabarraei, A, Yazdani, Y,

Abstract Background and objective: Hepcidin is a cystein-rich antimicrobial peptide, which is secreted by the liver. It fights against wide spectrum of bacteria, viruses and fungi and it is a major regulator of iron homeostasis. Today, scientists have made many efforts on the production of hepcidin. Baculovirus expression system is one of the best eukaryotic expression systems for production of...

Journal: :iranian biomedical journal 0
نوشین داوودی noushin davoudi فریدون مهبودی fereidoun mahboudi محمد عزیزی mohammad azizi احمد عادلی ahmad adeli robert. w mcmaster

the ple2scx vector was developed for the stable expression of exogenous genes in the protozoan parasite leishmania. the ple2scx construct contains three independent selection markers: herpes simplex virus thymidine kinase (hsv-tk), cytosine deaminase (cd) and streptothericin acetyltransferase gene (sat) in multiple cloning site, flanking by 5’ and 3’ untranslated regions of the previously clone...

Background and Aim: As one of the factors of gastric ulcers and cancer, Helicobacter pylori can live in the acidic environment of stomach for many years due to having urease enzyme. This enzyme requires Ni2+ and a group of auxiliary proteins such as ureE for its catalytic activity. Urease is not only a requisite factor to colonize the Helicobacter pylori but it is also pathogenic with diff...

Journal: :reports of biochemistry and molecular biology 0
atieh yaghoubi antimicrobial resistance research center, mashhad university of medical sciences, mashhad, iran ehsan aryan antimicrobial resistance research center, mashhad university of medical sciences, mashhad, iran hosna zare antimicrobial resistance research center, mashhad university of medical sciences, mashhad, iran shadi alami antimicrobial resistance research center, mashhad university of medical sciences, mashhad, iran roghayeh teimourpour department of microbiology, school of medicine, ardabil university of medical sciences, ardabil, iran. zahra meshkat tel: +98 51 38012453; fax: +98 51 38002960

background: tuberculosis (tb) is a major cause of death worldwide. finding an effective vaccine against tb is the best way to control it. several vaccines against this disease have been developed but none are completely protective. the aim of this study was to design and construct a cloning vector containing the mycobacterium tuberculosis (m. tuberculosis) heat shock protein x (hspx). methods: ...

نمودار تعداد نتایج جستجو در هر سال

با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید