We describe two fully enzymic methods, fluorometric and colorimetric, for determination of triglycerides (triacylglycerols) in serum. Samples are incubated with microbial lipase for 10 min, and the glycerol released from the triglycerides is oxidized by NAD+ in the presence of glycerol dehydrogenase. In the fluorometric method, the resulting NADH is in turn oxidized by resazurin as catalyzed by...

A colorimetric method for quantitative measurement of the susceptibility of Mycobacterium tuberculosis to antimicrobial agents is described. The method utilizes an oxidation-reduction dye, Alamar blue, as an indicator of growth. By this method, MICs of isoniazid, rifampin, streptomycin, and ethambutol were determined for 50 strains of M. tuberculosis. Colorimetric MIC results were available on ...

We have evaluated three analytical methods for determining inorganic phosphorus in serum applied to the Technicon RA-I000 analyzer: a fully enzymatic colorimetric method based on the specific system purine nucleoside phosphorylase/xanthine oxidase coupled to an indicator colorimetric reaction similar to the Trinder reaction; a chemical method involving the direct UV measurement of the phosphomo...

exist for cholinesterase assay, but each has drawbacks (1, 2). This paper presents an improved version of Garry and Routh's modification (3) of Ellman's method, as well as variations of this method for the assay of " true " cholinesterase in red cells and for the determination of the dibucaine number. The basic reactions are: Milwaukee, Wis. 53233), 2.0 mg/mI in 0.2 molar potassium phosphate bu...

Menthol vapours are irritant to eyes, nose and throat, and ingestion of this chemical has been reported by Von Oetingen (1958)1) to cause abdominal pain, vomiting, staggering gait and sopor. The workers exposed to high concentrations of menthol vapours in an industry manufacturing menthol crystalls from the plant extracted mentha oil complained of drowsiness, headache, nausea and vomiting. Till...

In this paper, methods of sample preparation for potentiometric measurement of phenylalanine are presented. Basing on the spectrophotometric measurements of phenylalanine, the concentrations of reagents of the enzymatic reaction (10 mM L-Phe, 0,4 mM NAD, 2U L-PheDH) were determined. Then, the absorption spectrum of the reaction product, NADH, was monitored (maximum peak at 340 nm). The results ...