BACKGROUND Corneal endothelial damage is a known complication of aqueous shunt surgery. OBJECTIVE To describe a new technique for repositioning the Ahmed glaucoma valve tube in a case of tube-endothelial touch. CASE A patient with advanced glaucoma, having undergone Ahmed glaucoma valve (AVG) implantation, developed localized corneal endothelial damage due to contact between the tube and su...

Purpose: The evaluation of the corneal endothelium patients with pseudoexfoliation syndrome (PES) by specular microscopy (SM). Method: In this prospective study, two groups were formed. our right eye 50 diagnosed material slit lamp biomicroscopy performed in ophthalmology outpatient clinic and control without any health problems included. Results: There was no difference between patient terms g...

Human cornea is a highly organized transparent tissue and is well determined by its functional layers as shown in (Figure 1). The posterior layer of the cornea is the endothelium which is a physiologically important monolayer of cells. Human Corneal Endothelium (CE) plays a major role in maintaining the corneal transparency, thickness and hydration [1,2]. As CE dysfunction is the second leading...

Clinical-grade ex vivo expansion of corneal endothelial cells can increase the availability of corneal tissues for transplantation and treatment of corneal blindness. However, these cells have very limited proliferative capacity. Successful propagation has required so far to use very complex growth media supplemented with fetal bovine serum and other xenocomponents. We hypothesized that human p...

PURPOSE To compare the relative expression of peroxiredoxin (Prx) proteins in normal human corneal endothelium with endothelium in corneas affected by Fuchs' endothelial dystrophy (FED) and between normal human endothelium and epithelial/stromal tissue. METHODS Human corneal endothelial cell-Descemet's membrane (HCEC-DM) complexes from normal and FED corneal buttons were dissected from the ep...

The cornea is composed of a multilayered epithelium, Bowman's membrane, stroma, Descemet's membrane, and endothelium. The corneal endothelium (CE) is a single layer of hexagonal cells that separates the corneal stroma from the aqueous humor of the anterior chamber. Transparency of the cornea is maintained by regulation of stromal hydration through the barrier and pump functions of the CE, and c...

In response to stress, the corneal endothelium must maintain or regain its barrier function. To study cellular responses of the corneal endothelium, our laboratory has developed an in vitro model of rabbit corneal endothelial wound closure. When cells are free to divide, a 3 mm diameter wound closes within 4 days. 5-fluorouracil added to these cultures does not affect the cellular morphology or...

In response to stress, the corneal endothelium must maintain or region its barrier function. To study cellular responses of the corneal endothelium, our laboratory has developed an in vitro model of rabbit corneal endothelial wound closure. When cells are free to divide, a 3 mm diameter wound closes within 4 days. 5-fluorouracil added to these cultures does not affect the cellular morphology or...