The prevalence of bovine viral diarrhoea virus (BVDV) biotypes and subgenotypes was determined from 131 BVDV positive samples from a diagnostic laboratory. The majority of the isolates were from Oklahoma; however, other states including Kansas, Texas, and Arkansas were represented. These BVDV samples were from submissions of 76 live animals and 55 necropsy samples. There were 131 BVDV samples r...

One of the most efficient mechanisms by which plants protect themselves from invading virusesis the specific RNA-dependent silencing pathway termed post-transcriptional gene silencing(PTGS). In this mechanism, resistance to a virus is engineered through the expression of asegment of the virus genomein transgenic plants. Potato VirusY (PVY) is one of the mostdamaging viruses of potato, infecting...

Silencing XB130 inhibits cell proliferation and epithelial‑mesenchymal transition in non‑small lung cancer (NSCLC), suggesting that downregulating expression may impede NSCLC progression. However, the molecular mechanism underlying regulation of remains unclear. In present study, role 3'‑untranslated region (3'‑UTR) was investigated. Recombinant psiCHECK‑2 vectors with wild‑type, truncated, or ...

objective(s):oct4 is a transcription factor required for pluripotency during early embryogenesis and the maintenance of identity of embryonic stem cells and pluripotent cells. therefore, the effective expression regulation of this gene is highly critical. utr regions are of great significance to gene regulation. in this study, we aimed to investigate the potential regulatory role played by 5´ut...

The four encapsidated RNAs of brome mosaic virus (BMV; B1, B2, B3, and B4) contain a highly conserved 3' 200-nucleotide (nt) region encompassing the tRNA-like structure (TLS) which is required for packaging in vitro (Y. G. Choi, T. W. Dreher, and A. L. N. Rao, Proc. Natl. Acad. Sci. USA 99:655-660, 2002). To validate these observations in vivo, we performed packaging assays using Agrobacterium-...

Cassava brown streak disease (CBSD) has occurred in the Indian Ocean coastal lowlands and some areas of Malawi in East Africa for decades, and makes the storage roots of cassava unsuitable for consumption. CBSD is associated with Cassava brown streak virus (CBSV) and the recently described Ugandan cassava brown streak virus (UCBSV) [picorna-like (+)ssRNA viruses; genus Ipomovirus; family Potyvi...

Rapid detection and differentiation of infectious bronchitis virus (IBV) involved in the disease outbreak is very important for controlling disease and developing new vaccines. In the present study, three regions of the genome of IBV vaccine and field isolates including S1 gene, gene 3 and nucleocapsid (N) gene along with 3' untranslated region (3' UTR) were amplified and subjected to restricti...

The present study was conducted on the Nile tilapia (Oreochromis niloticus) and screened for single nucleotide polymorphisms (SNPs) in muscle hormone genes myogenin (MyoG) myostatin (MSTN). Samples were collected from JangHang fish farm categorized by body weight sex. average total 1.02 kg 82 males 0.63 70 females. A range of ±10% considered as normal weight. Target regions each gene categorize...