The complete amino acid sequences of [2Fe-2S] ferredoxins from Scopolia japonica and Lycium chinense have been determined by automated Edman degradation of the entire Cm-proteins and of the peptides obtained by enzymatic digestions. These two ferredoxins exhibited only 2-7 differences in the amino acid sequence when compared to the Datura-ferredoxins (D. stramonium, D. metel, and D. arborea), a...

The antimycotic potential of Datura metel (syn. Datura alba Nees.) was investigated in vitro against Ascochyta rabiei, the cause of chickpea blight disease. The pathogen was exposed to various n-hexane concentrations (1, 2, 3 and 4% w/v) of shoot and root extracts of D. metel using poisoning food technique. All the employed concentrations of both root and shoot extracts significantly suppressed...

Poisoning of a young adolescent caused by the intake of extracts prepared from the plant Datura stramonium is presented. It is a broadleaf annual erect herb with spine covered seed capsule. Its toxic alkaloids, atropine and scopolamine, cause a series of characteristic classic symptoms of anticholinergic poisoning. Children are mostly exposed to the poisoning by the plant species from the genus...

Fifty three alkaloids were identified in the organs (roots, stems, leaves, flowers, and seeds) of Datura innoxia by GC/MS. Seventeen of them are reported for the first time for this species and one nor-derivative, 3-phenylacetoxynortropane (28), for the genus Datura. Furthermore, four new tropane esters were tentatively identified as 3-acetoxy-6,7-epoxytropane (acetylscopine) (10), 3-acetoxy-6-...

Aqueous leaf extract of four different species of Datura viz. D.stramonium L., D. innoxia Mill.Gard, D. metal L. and D. ferox L. Amoen were tested for their antifungal activity against phytopathogenic molds in terms of mycelial growth of Alternaria solani and Fusarium oxysporum f.sp. udum causing early blight of tomato and wilt of pigeonpea respectively. The extract of Datura sp. at 5, 10, 15 a...

Three flavonoidal aglycones viz; F1 (Chrysin), F2 (Kampferol) and novel F3( 3,7dimethylether quercetin), were isolated from acetone wash of fresh leaves of Datura stramomium L. Isolated compounds were identified on the basis of their physico-chemical properties, Co-TLC, H-NMR, C-NMR and CIMS specrtum. Significant cytotoxic activity against three tumor cell lines (liver, cervix & breast), was re...

plant tissue culture techniques were used to study the effect of nacl on tropane alkaloids accumulation and proline content of both datura metel and datura stramonium. callus cultures were established for both datura species. however, ms medium supplemented with 1mg/l of both ba and naa was the best for callus production in d. metel, while ms supplemented with 3 mg/l kin and 1 mg/l 2, 4-d was t...