Tritium-labeled digitoxin, digitoxigenin, digoxin, and digoxigenin of established purity and chemcal authenticity were used to study the binding of these compounds to human plasma proteins. 97% of digitoxin in plasma was nondialyzable. Continuous flow paper electrophoresis of plasma containing digitoxin and dialysis experiments in which human serum albumin competed for the glycoside with plasma...

A study of the urinary excretion of digitoxin in man was first attempted by Friedman and coworkers (1) by the use of the embryonic duck heart method of biological assay. These workers estimated that the amount of cardioactive substances (calculated as digitoxin) excreted in the urine of young adults over a period of 12 to 24 days after an oral dose was equivalent to approximately 40 per cent of...

3H-digitoxin and polyethylene glycol (nonabsorbable marker) were given orally to five subjects provided with a gastrointestinal tube. Of the radioactivity administered, approximately 15, 30, and 70% had been absorbed when the test solution was passing the stomach, the duodenum, and the upper jejunum, respectively. The absorption was rapid, and the plasma contained detectable amounts of radioact...

This work was presented in part at the 62nd Annual Meeting of the American Society for Clinical Investigation, May 1970, Atlantic City, N. J. Received for publication 16 September 1970 and in revised form 12 August 1971. The data obtained in this study indicate that cholestyramine treatment affords a significant degree of protection against lethal digitoxin intoxication in rats and guinea pigs....

Following the introduction of the embryonic duck heart assay method (1), and the radioactive isotope tracer technique (2), means were available for the quantitative study of the renal excretion of various cardioactive glycosides in both the experimental animal and man. It was but natural that the renal excretion of the digitalis glycoside, digitoxin, received the first attention of both the inv...

" ¡–ì • ŸŽq • E 'åŽ-‰p • ¢ • E Ž • " c —S • G • E " c' † OEì Though the view, that there would be an intimate relationship between the cardiac action of digitalis glycosides and the uptake of the drugs by heart, has been often regarded as a well established basis in understanding of the mechanism in the cardiac action of the drugs (1, 2), direct evidence for its validity is not yet given. The p...

We measured the cross reactivity of two medications--digitoxin and spironolactone--in two digoxin radioimmunoassay (liquid and solid-phase) kit procedures. Both tests showed similar average percentages of cross reactivity with digitoxin (7.2 and 8.9% for intravenous, and 11.9 and 10.9% for oral administration), but no cross reactivity with spironolactone or its metabolites after equal intraveno...

There is little known about the nature of the metabolic products derived from cardiac glycosides in the animal body. Reports of the formation of digitoxigenin from the glycoside digitoxin in vivo have appeared in the literature (l-3), but reliable chemical methods of identification applicable to the very small quantities of material involved were not available to these workers. Fischer, Sjoerds...