BACKGROUND Keeping in mind the commercial application of polygalacturonase (PG) in juice and beverages industry, bacterial strains were isolated from rotten fruits and vegetables to screen for competent producers of PG. OBJECTIVES In this study, the plate method was used for preliminary screening of polygalacturonase-producing bacteria, while the Dinitrosalicylic Acid (DNS) method was used fo...

A development of a technique for estimating sugar in blood, cerebrospinal fluid, etc., is described, using 3:5-dinitrosalicylic acid (D.N.S.A.) originally introduced by Sumner (1921). Results can be obtained in less than 10 minutes if required. The method is well suited to the estimation of random blood sugars and the handling of diabetic clinic requirements in hospital laboratories. The reagen...

Plant expression of thermostable endoglucanase (E1) [pdb:1ECE] has been proposed for improved conversion of lignocellulose to ethanol for fuel production. Since E1 was isolated from hot springs and has thermostable properties, it may have increased stability when released to agricultural soils during post-harvest residue decomposition. Endoglucanases are a subclass of cellulases and act by clea...

Enzyme-retting of flax was accomplished via individual treatment with four polygalacturonase (PGase) containing solutions of various fungal sources and the resulting fibers were characterized. The retting solutions were equilibrated to contain 2.19 U of PGase activity as determined via a dinitrosalicylic acid (DNS) reducing sugar assay. As compared with the buffer control, treatment with the va...

The effects of hydrolysis methods (hydrothermal, acid, alkali, hydrothermal-enzyme, acid-enzyme, and alkali-enzyme) on hydrolysate characteristics photo fermentative hydrogen production (PFHP) corn straw (CS) sorghum (SS) were investigated. optimum reducing the sugar in different solvent environments was studied by one-step co-enzymatic pretreatment through a 3,5-dinitrosalicylic acid method. p...

BACKGROUND Moringa stenopetala has been used in traditional health systems to treat diabetes mellitus. One of the successful methods to prevent of the onset of diabetes is to control postprandial hyperglycemia by the inhibition of α-glucosidase and pancreatic α-amylase activities, resulting in the aggressive delay of the carbohydrate digestion of absorbable monosaccharides. The aim of the prese...