Diosmetin, 3',5,7-trihydroxy-4'-methoxy flavone, is the aglycone of the flavonoid glycoside diosmin that occurs naturally in foods of plant origin. Diosmin exhibits antioxidant and anti-inflammatory activities, improves venous tone and it is used for the treatment of chronic venous insufficiency. Diosmin is hydrolyzed by enzymes of intestinal micro flora before absorption of its aglycone diosme...

Maternal immune activation (MIA) can affect fetal brain development and thus behavior of young and adult offspring. Reports have shown that increased Interleukin-6 (IL-6) in the maternal serum plays a key role in altering fetal brain development, and may impair social behaviors in the offspring. Interestingly, these effects could be attenuated by blocking IL-6. The current study investigated th...

A new colorimetric method for determination of diosmin is proposed. The method is based on formation of a chromophore by the flavonoid in presence of 75% (v/v) HCl showing maximum absorbance at 405 nm. The technique with linearity over 10 through 80μg flavonoid (r ± s.e. = 0.9994 ± 0.0008, b±s.e. = 0.0089 ± 0.0001, COV ± s.e. = 2.8 ± 0.5, n= 6 each) has been compared with standard aluminium chl...

The polyphenolic distribution of Vitis vinifera seeds (GSE), Citrus spp. fruits (CE) and Olea europaea L. leaves (OL) extracts were characterized and quantified by HPLC methods. Radioprotective effects of these extracts and the reference flavonoids diosmin and rutin, widely used as pharmaceuticals, were determined by using the micronucleus test for anticlastogenic activity, evaluating the reduc...

High-performance liquid chromatography-diode array detector (HPLC-DAD) coupled with electronspray mass spectrometry (ESI-MS) was used to detect the flavonoid profile in lemon juices obtained from the main Sicilian cultivars (Femminello comune, Monachello, and Interdonato). Significant amounts of an unusual constituent were found in the lemon juice of the above-mentioned cultivars together with ...

A simplified version of the 2, 4-DNPH assay for flavonoids has been improvised. The assay includes incubating test sample with DNPH in a 4.5 mL reaction volume in hot water at about 80 C containing 2 mL methanol at about 0.2 N HCl, cooling samples to room temperature, adding 3 mL 1M NaOH, and monitoring red color at 470 nm at 10-15 minutes. The reagent has been found capable of assaying both fl...

Diosmin is a flavonoid often administered in the treatment of chronic venous insufficiency, hemorrhoids, and related affections. Diosmin is rapidly hydrolized in the intestine to its aglicone, diosmetin, which is further metabolized to conjugates. In this study, the development and validations of three new methods for the determination of diosmetin, free and after enzymatic deconjugation, and o...

Background and purpose: Fear of pain is one of the main reasons for refusing surgical hemorrhoidectomy among patients with hemorrhoids. This study investigated the effect of Diosmin on post-hemorrhoidectomy pain. Materials and methods: A single-blind randomized clinical trial was performed in 92 patients undergoing hemorrhoidectomy. They were randomly divided into two groups; the intervention ...

For poorly soluble drugs, the digestive absorption depends on their rate of dissolution. Decreasing the particle size of these drugs improves their rate of dissolution. Fine grinding mills are use to micronize powders: either jar mills or fluid energy mills. Theses processes were applied to griseofulvin, progesterone, spironolactone and diosmin. For each drug, micronization improved their diges...