introduction: follicle stimulating hormone (fsh) is one of the pituitary glycoproteines that it consists of two subunits alpha and beta. the beta subunit is responsible for the biological activity of fsh. the aim of present study was isolation of the β subunit coding sequence containing its signal sequence from human genome and then cloning of the isolated sequence in ppic9 shuttle vector under...

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genetic typing methods of t. gondii strains have been extensively perfected in recent years. from a technical point of view, many tools usable for genetic studied on single-copy loci have been used: rflp, pcr-rflp, sequencing, rapd-pcr and isoenzyme analysis. we described the cloning and sequence analysis of the gene which encodes the major surface antigen (sag1 or p30) of t. gondii. sag1 is th...

The emergence of hybrid DNA–protein nanostructures in recent years has expanded the application DNA nanotechnology. Previous studies reported integration proteins into by sequence-dependent interactions or chemical modifications DNA, which limit design flexibility nanostructures. Here we report construction sequence-independent geometry-based using self-assembly single-stranded (ss) and histone...

Genomic DNA extraction with a high quantity and quality is a basic requirement in molecular biology. The DNA obtained was free of any contamination proteins, polysaccharide, polyphenols and colored pigments. These compounds would interfere with the genomic isolation procedures and downstream reactions such as restriction enzyme analysis and gene amplification. The isolated genomic DNA was fo...

differentiation of stem cells from a pluripotent to a committed state involves global changes in genome expression patterns, the best known as “epigenetic modifications”. epigenetic mechanisms, including histone modifications, dna methylation and non-coding rna-mediated regulatory events are considered as the essential factors to control the heritable cellular memory of gene expression during d...