Five different immunoassay formats were examined for their ability to detect a minute quantity of prey remains in predator guts. The convergent lady beetle, Hippodamia convergens Guérin-Meneville, that had consumed either one or five pink bollworm, Pectinophora gossypiella (Saunders), eggs was evaluated by the following immunoassays: three variations of enzyme-linked immunosorbent assay (ELISA)...

Rice tungro disease (RTD) is one of the most destructive diseases of rice in South and Southeast Asia. RTD is routinely detected based on visual observation of the plant. However, it is not always easy to identify the disease in the field as it is often confused with other diseases or physiological disorders. Here we report the development of two serological based assays for ease of detection o...

Background and Objective: Today, one of the problems of health systems is the presence of cockroaches in hospitals as insects that move freely in and out of the hospitals and are infected with pathogenic bacteria. The aim of this study was to identify carbapenem resistance genes in Escherichia coli isolated from Blattella germanica by dot blot assay in Hamadan hospitals in the west of Iran. Me...

We investigated two techniques of immunoblotting--the Western blot and the dot blot--for use in detecting prostatic acid phosphatase (PAP, EC 3.1.3.2). We used polyclonal antisera to human PAP, produced in rabbits by hyperimmunization with purified PAP, and PAP-specific monoclonal antibodies in the immunoenzymatic protocols. We conclude that PAP can be readily detected by Western blots with use...

We report the potential use of a specific Toxoplasma gondii DNA probe (ABGTg7). We applied a dot blot hybridization assay to blood samples for the diagnosis of cerebral toxoplasmosis (CT), acute toxoplasmic lymphadenopathy (ATL), and disseminated toxoplasmosis in transplant recipients (TRs). We studied a total of 84 individuals: 38 patients and 46 controls. We found positive hybridization signa...