نتایج جستجو برای: elisa technique
تعداد نتایج: 653357 فیلتر نتایج به سال:
growing demands and requires of high data rate systems cause significant increase of high frequency systems for wideband communication applications. as mixers are one of the main blocks of each receivers and its performance has great impact on receiver’s performance; in this thesis, a new solution for ku-band (12-18 ghz) mixer design in tsmc 0.18 µm is presented. this mixer has high linearity a...
A dot-ELISA test for the detection of anti-BRSV antibodies is described. The objective of this study was the standardisation of a test as a fast, inexpensive and effective alternative to detect anti-BRSV antibodies. Its sensitivity, specificity and usefulness were compared to a commercial ELISA-kit and to the standard serum neutralisation (SN) test. The standardisation of the technique was done...
Toxoplasmosis is a zoonosis of broad geographic distribution. This disease is caused by infection with the protozoan parasite Toxoplasma gondii. The most important ways to diagnose the disease are the serological techniques such as IFA and ELISA. The aim of this consideration, was comparison between the two methods, IFA and ELISA, in order to determine the incidence of antibody against Toxoplas...
I. Introduction ELISA, enzyme-linked immunosorbent assay, is a biochemical technique used for detecting specific compound(s) in environmental and biological samples. The technique has been successfully applied for analyses of pesticides in soil and water samples by the also evaluated commercial diazinon and chlorpyrifos ELISA kits for their reliability and usefulness for surface water samples c...
On the basis of partial amplification of a cloned fragment of kDNA of Leishmania infantum which is specific for this species, we developed a PCR-ELISA technique which avoids the problems associated with classical diagnostic techniques. This technique was tested on 33 L. infantum strains from 19 different zymodemes, which were recognized equally. It was also used on human and canine clinical sam...
Three methods were compared for the detection of prunus necrotic ringspot virus in herbaceous and woody plants: DAS-ELISA, nonisotopic dot-blot hybridization and reverse transcriptional polymerase chain reaction (RT-PCR). When purified virus preparations were used, the detection limit of the RT-PCR technique was 1·28 pg mL whereas nonisotopic molecular hybridization and DAS-ELISA allowed detect...
Following an outbreak of fascioliasis in Anzali Harbor in 1988, 452 blood samples were collected using a multistage sampling method for hematological and serological studies. After determination of eosinophilia in the samples, antifasciolia antibodies in the sera were also determined using enzyme linked immuno sorbant assay (ELISA) and counter-current immuno electrophoresis (CCIE). 64.2% ...
BACKGROUND Veterinary drugs such as clenbuterol (CL) and sulfamethazine (SM2) are low molecular weight (<1000 Da) compounds, or haptens, that are difficult to develop immunoassays due to their low immunogenicity. In this study, we conjugated the drugs to ovalbumin to increase their immunogenicity for antiserum production in rabbits and developed a protein microarray immunoassay for detection of...
A new Enzyme Linked Immuno Sorbent Assay (ELISA) kit for the determination of serum ferritin has been compared with another ferritin kit based on the Immuno Radio-Metric Assay (IRMA) approach, both assays containing similar antibodies. Based on these studies, we found the within-run precision of the ELISA (and IRMA) to have coefficients of variation of 4-10% and 2-6% respectively, over a concen...
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