AIMS To examine the relation between enteroviral infection, especially group A coxsackieviral infection, and acute febrile illness over two summers using tissue culture and polymerase chain reaction (PCR). METHODS Throat swabs were collected from 246 children from June to August 1997 and 1998. RESULTS Enteroviruses were isolated from 33/246 samples and 35 other viruses were isolated. Entero...

mumps infection is endemic in iran. our objective was to evaluate the presence of anti mumps antibodies ( igm & igg) in cerebrospinal fluid in mumps meningoencephalitic children. a prospective/cross-sectional study was performed in tehran, iran (2003 to 2004) and serum anti mumps antibodies (igm) were detected (quantitive; elisa) in meningoencephaltis patients. specific anti mumps antibodies (i...

Results We identified 169 children treated for refractory AID: autoimmune cytopenia (s) (104 patients), systemic lupus erythematosus (SLE) (52 patients), miscellaneous (13 patients). The mean follow-up period was 6 to 36 months. Patients received 2 to 4 rituximab infusions (350–750 mg/m2) associated with immunosuppressive drugs in 49/ 52 SLE patients. Replacement intravenous immunoglobulins the...

The polymerase chain reaction (PCR) is a new diagnostic technique for the detection of enteroviral infection; however, it currently provides only qualitative results. The aim of this study was to adapt PCR for the accurate quantitation of enteroviral RNA in clinical specimens. For this purpose, we designed a standard RNA which was homologous to sequences at the 5' end of the coxsackie B3 entero...

Amyotrophic lateral sclerosis (ALS) is a devastating neurodegenerative disease that primarily attacks motor neurons in the brain and spinal cord, leading to progressive paralysis and ultimately death. Currently there is no effective therapy. The majority of ALS cases are sporadic, with no known family history; unfortunately the etiology remains largely unknown. Contribution of Enteroviruses (EV...

We have developed an in situ hybridization assay capable of detecting enteroviral RNA in myocardial cells, using molecularly cloned coxsackievirus B3 cDNA as a diagnostic probe. Because of the high degree of nucleic acid sequence homology among the numerous enteroviral serotypes, including the group A and B coxsackieviruses and the echoviruses, detection of these various agents commonly implica...

OBJECTIVES The aim of this study was to evaluate the viral etiology of idiopathic dilated cardiomyopathy (DCM). BACKGROUND The demonstration of enteroviral genome in hearts with DCM has reinforced the importance of enteroviruses in the pathogenesis of DCM. However, there is uncertainty about the character and activity of enteroviruses detected in the myocardium. Recently, the association of h...

Background and Objectives: Meningoencephalitis in Iranian children is frequent, but encephalitis is rare .The frequency of HHV-6 and HHV-7 in central nervous system diseases of our children is unclear. The aim of this study was searching the DNA-s of HHV-6 & HHV-7 in CSF samples of children with meningoencephalitis  Materials and Methods: A cross sectional study (2007-2009) was done in Pediatr...