background: aflatoxin m1 (afm1) as a carcinogenic and toxic compound has considerable significance from public health viewpoint. the aim of this study was to determine the level of afm1 in pasteurized and uht milk in west-azerbaijan province of iran. methods: a total of 360 commercial milk samples including 220 pasteurized milk and 140 ultra-high-temperature (uht) milk were purchased randomly f...

cholera toxin b subunit (ctb) has been extensively studied as an immunogen, adjuvant, and inducer of oral tolerance in many investigations. production of ctb has been carried out in the bacterial, plant, insect and yeast expression systems. in this study the expression of the ctb containing a 6xhis-tagged was performed by escherichia coli (e.coli) m15. the yield of purified pentameric recombina...

results the optimal conditions were considered to perform elisa. comparison between results obtained from optimized elisa kit and commercial elisa kit showed a good agreement. background haemophilus influenzae type b (hib) infection has high morbidity and mortality rate, especially in children under 5 years of age. enzyme-linked immunosorbent assay (elisa) technique is the most used method to d...

there are many studies related to the production of a elisa kit for diagnosing virus infections. however, production of most kits depends on purification of whole virus particles, which involves the use of costly equipment and reagents. the purpose of this study was to check out if the anti-cp42 antibodies could be used as a diagnostic assay for detection of grapevine fanleaf virus (gflv). in t...

diagnosis of toxoplasma gondii (t.gondii) infection is of great medical importance especially for pregnant women and immunosuppressed patients. numerous studies have shown that the recombinant production of several ttoxoplasma antigens, including dense granule antigens (gras) has a great potential as diagnostic reagents. previous studies reported expression of amino terminal gra8 protein in fus...