Abstract The outbreak of the new severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is a public health emergency. Asthma does not represent risk factor for COVID-19 in several published cohorts. We hypothesized that SARS-CoV-2 proteome contains T cell epitopes, which are potentially cross-reactive to allergen epitopes. aimed at identifying homologous peptide sequences by means two dis...

Background : Production of monoclonal antibodies to Leishmania antigens assists the identification and characterization of these organisms. Objective: Production of monoclonal antibodies against epitopes on the gp63. Methods: Two murine monoclonal antibodies to gp63 were produced and characterized. The reactions of both antibodies with soluble leishmanial antigens, purified gp63 and truncated r...

Objective(s):Brucellosis is a well-known domestic animal infectious disease, which is caused by Brucella bacterium. GroEL antigen increases Brucella survival and is one of the major antigens that stimulates the immune system. Hence, the objective of the present study was cloning and bioinformatics analysis of GroEL gene. Materials and Methods: The full-length open reading frame of this gene was...

Background: Hepatitis C virus (HCV) causes acute and chronic human hepatitis infections. Due to the high genetic diversity and high rates of mutations in the genetic material so far there is no approved vaccine against HCV. Materials and Methods: The aim of this study was to determination B and T cell conserved epitopes of E1 and E2 proteins from HCV and construction of a chimeric pepti...

OBJECTIVE To identify and characterize cytotoxic T-cell (CTL) epitopes for HIV-1 clade E using eight known HLA-A*1101-restricted HIV-1 clade B epitopes. METHODS Induction of clade E-specific CTL was examined by stimulating peripheral blood mononuclear cells (PBMC) from clade E-infected Thai individuals with the clade E-specific peptide corresponding to the clade B epitopes. Cross-clade and cl...

Background: Contraceptive vaccines (CVs) can be used as a valuable and alternative method for the prevention of gestation in humans and animals. These vaccines can have several targets, such as superficial sperm proteins. Vaccines based on sperm antigens are quite efficacious to create a contraceptive effect. However, multi-epitope vaccines are more effective in stimulating the immune system an...

Development of efficient peptide-based immunotherapy for shrimp allergy relies on the identification dominant T-cell epitopes its major allergen, tropomyosin. In this study, immunoinformatic tools, proliferation, cytokine release, IgG/IgE binding, and degranulation assays were used to identify characterize in Lit v 1 comparison with previously validated B-cell epitopes. The results showed that ...

Antibodies against Mycoplasma gallisepticum (M. gallisepticum) block the antigenic epitopes of bioaptamer-binding to PvpA protein, resulting in a lighter colorimetric detection.

conclusions hcv gpe2 is an ideal target for hcv vaccine. prediction of epitope immunogenicity and characterization on the basis of peptide sequences will be significantly helpful for development of a heterologous vaccine against hcv variants. results many b-cell and t-cell epitopes predicted in gpe2 were found conserved among hcv 3a genotypes whereas few were conserved in other genotypes antici...

One of the major challenges in designing a peptide-based vaccine is the identification of antigenic regions in an antigen that can stimulate B-cell's response, also called B-cell epitopes. In the past, several methods have been developed for the prediction of conformational and linear (or continuous) B-cell epitopes. However, the existing methods for predicting linear B-cell epitopes are far fr...