Optimization of production medium is required to maximize the metabolite yield. This can be achieved by using a wide range of techniques from classical "one-factor-at-a-time" to modern statistical and mathematical techniques, viz. artificial neural network (ANN), genetic algorithm (GA) etc. Every technique comes with its own advantages and disadvantages, and despite drawbacks some techniques ar...

In this study we determined the influence of different sugar concentration in media, time of rehydration and type of strain on relative expression level of GPD1 and SIP18 genes of active dry cider-making yeast strains, followed by the assessment of the impact of rehydration on the fermentation process. High expression of SIP18 at the beginning of rehydration was shown to be due to high transcri...

Natural fermentation of shredded carrots using two types of carrots (red and crimson coloured) was carried out with 3% salt and 4% mustard powder. The viable number of microorganisms was determined at various time intervals on four different types of media viz. De Man Rogosa Sharpe (MRS), Nutrient agar, Yeast extract peptone dextrose agar (YEPDA) and Acetobacter medium. The viable number of cel...

Nowadays, vinegar is industrially produced by mesophilic acetic acid bacteria (AAB). However, temperature fluctuation during acetous fermentation is inevitable, and may cause process disturbances. This can be mostly avoided using thermo-tolerant AAB. The main purpose of the present study was to isolate thermo-tolerant AAB. Fermentation performances were then evaluated. Twenty-eight different is...

Agro-industrial wastes are mainly composed of complex polysaccharides that might serve as nutrients for microbial growth and production of enzymes. The aim of this work was to study polygalacturonase (PG) production by Aspergillus niveus cultured on liquid or solid media supplemented with agro-industrial wastes. Submerged fermentation (SbmF) was tested using Czapeck media supplemented with 28 d...

Effects of xylose on xylanase production by a thermophilic Bacillus sp showed diverse patterns on corn cob (CC) and wheat bran (WB) as sole carbon sources in solidstate fermentation (SSF) and submerged fermentation (SmF). Supplementation of these media with either mineral salt solution (MSS) or yeast extract peptone (YEP) also exerted variable effects. While under SSF, xylose stimulated xylanas...

Microbial cells are producers of natural polymers present in plant cells. Production of pullulan (an extracellular microbial polysaccharide) by Aureobasidium pullularia pullulans (P. pullulans) was studied under fermentation conditions, and kinetic parameters were determined. Pullulan formation obeyed a growth and non-growth associated term. PHB (polyhydroxybutyrates) an intracellular biopo...

The utilization of fermentation media derived from waste and by-product streams from biodiesel and confectionery industries could lead to highly efficient production of bacterial cellulose. Batch fermentations with the bacterial strain Komagataeibacter sucrofermentans DSM (Deutsche Sammlung von Mikroorganismen) 15973 were initially carried out in synthetic media using commercial sugars and crud...

BACKGROUND Elastin-like polypeptides (ELPs) are useful tools that can be used to non-chromatographically purify proteins. When paired with self-cleaving inteins, they can be used as economical self-cleaving purification tags. However, ELPs and ELP-tagged target proteins have been traditionally expressed using highly enriched media in shake flask cultures, which are generally not amenable to sca...

microbial cells are producers of natural polymers present in plant cells. production of pullulan (an extracellular microbial polysaccharide) by aureobasidium pullularia pullulans (p. pullulans) was studied under fermentation conditions, and kinetic parameters were determined. pullulan formation obeyed a growth and non-growth associated term. phb (polyhydroxybutyrates) an intracellular biopolyme...