The population dynamics of pseudomonads in gilt-head sea bream Mediterranean fish (Sparus aurata) stored under different conditions were studied. Phenotypic analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins were performed to identify a total of 106 Pseudomonas strains isolated from S. aurata stored under different temperatures (at 0, 10, and 20 degree...

This chapter aims to describe the early stages of biofilm formation, particularly on abiotic surfaces, by focusing on Pseudomonas aeruginosa. Specifically, we will dissect the early steps in the establishment of a multicellular community: (i) translocation to the surface from a free-swimming planktonic lifestyle, (ii) initial or reversible attachment, and finally (iii) irreversible attachment. ...

The relationship of pyoverdins produced by 41 pathovars of Pseudomonas syringae and by phytopathogenic Pseudomonas species was investigated. A high-performance liquid chromatography method for analyzing the culture medium proved to be superior to isoelectric focusing for detecting pyoverdin production, for differentiating slightly different pyoverdins, and for differentiating atypical from typi...

Microorganisms produce iron chelators called siderophores that are a rich source for drug discovery or plant protective agents. Pyoverdines class of from fluorescent Pseudomonas members and consist different peptide chains specific to each bacterial species. The structural elucidation characterization pyoverdines require comprehensive analytical methods as extracts complex mixtures. Here, we pr...

Molecular evolution analysis of 16S rRNA sequences of native Pseudomonas strains and different fluorescent pseudomonads were conducted on the basis of Molecular Evolutionary Genetics Analysis version 5.2 (MEGA5.2). Topological evaluations show common origin for native strains with other known strains with available sequences at GenBank database. Phylogenetic affiliation of different Pseudomonas...

ABSTRACT Apple replant disease typically is managed through pre-plant application of broad-spectrum soil fumigants including methyl bromide. The impending loss or restricted use of soil fumigants and the needs of an expanding organic tree fruit industry necessitate the development of alternative control measures. The microbial community resident in a wheat field soil was shown to suppress compo...

Take-all decline (TAD) is a natural biological control of the wheat root disease “take-all” that develops in response to the disease during extended monoculture of wheat. The research to date on TAD has been mostly descriptive and no particular occurrence is yet fully understood. We demonstrate that root-associated fluorescent Pseudomonas spp. producing the antibiotic 2,4-diacetylphloroglucinol...