elucidating the critical epigenetics events involved in differentiation and reprogramming of cells to primordial germ cells (pgcs) is among the interesting issues in stem cell research. here, i will talk about critical transcription factors and global hypomethylation in development of germ cells. evidence strongly suggests that the earliest pgcs emerging in the e7.25 mouse embryo epiblast have ...

background: creation of artificial gametes may provide a universal solution for these patients of lacking gametes. stem cell technology may provide a way to obtain fully functional gametes. retinoic acid (ra) can initiate meiosis. several studies have demonstrated that ra can promote sperm cells differentiation from mouse embryonic stem cells (mescs) and other cells from human embryonic stem ce...

Teratomas are bulky lesions that rarely occur in the head and neck regions.They are composed of tissues from all germ layers with varying degrees of differentiation. They arise from pluripotential stem cells and ectopic embryonic non-germ cells. The most common sites of occurrence in the head and neck are the cervical region and the nasopharynx. Three cases of these tumors are reported here...

introduction: transplantation of germ cells restores the male fertility. nevertheless, a lot of questions remain incompletely resolved. the aim of this study was to evaluate in vitro colonization efficiency of germ cells and sperm production capacity of spermatogonial cells before and after culture by sperm number assay in epididymis of recipient mice. materials and methods: we developed a sert...

objective(s): the present day challenge is how to obtain germ cells from stem cells to treat patients with cancer and infertility. much more efforts have been made to develop a procedure for attaining germ cells in vitro. recently, human umbilical cord-derived mesenchymal stem cells (humscs) have been introduced with higher efficacy for differentiation. in this work, we tried to explore the eff...

Objective Genetic and morphologic similarities between mouse embryonic stem cell (ESCs) and Primordial Germ Cell (PGCs) make it difficult to distinguish the two cell types in in vitro differentiation. Using the expression of specific markers of germ cells that are not expressed or expressed at low levels in ESCs, can help recognizing in vitro differentiated cells MaterialsAndMethods In this stu...

teratomas are bulky lesions that rarely occur in the head and neck regions.they are composed of tissues from all germ layers with varying degrees of differentiation. they arise from pluripotential stem cells and ectopic embryonic non-germ cells. the most common sites of occurrence in the head and neck are the cervical region and the nasopharynx. three cases of these tumors are reported here tha...

objective(s):to culture thein vitro mouse embryonic stem cells (mescs) and to direct their  differentiation to germ-line cells; in present study we used a vector backbone containing the fusion construct stra8-egfp to select differentiated es cells that entered meiosis.  retinoic acid was used to differentiate embryonic stem cells to germ cells. materials and methods: a fragment of stra8 gene pr...