background and objectives: hyoscyamus reticulatus contains two distinguished tropane alkaloids, hyoscyamineand scopolamine and both of the compounds possesspotential acute or chronic toxicity. in the present study, a simple and efficient transformation system was established for in vitro hairy roots induction in hyoscyamus reticulatus. methods: effect of different factors including agrobacteriu...

Background and objectives: Hyoscyamus reticulatus contains two distinguished tropane alkaloids, hyoscyamineand scopolamine and both of the compounds possesspotential acute or chronic toxicity. In the present study, a simple and efficient transformation system was established for in vitro hairy roots induction in Hyoscyamus reticulatus. M...

A 12-year-old hairy root culture of Cichorium intybus L., a callus culture of the plant as well as roots and leaves of a wild plant of chicory, and roots of two C. intybus L. var. sativum cultivars were examined in respect of their hydroxycinnamate and sesquiterpene lactone compositions and contents. Total phenolics and diphenylpicrylhydrazyl radical scavenging activity of the examined plant ti...

Transformed hairy roots had been efficiently induced from the seedlings of Fagopyrum tataricum Gaertn. due to the infection of Agrobacterium rhizogenes. Hairy roots were able to display active elongation with high root branching in 1/2 MS medium without growth regulators. The stable introduction of rolB and aux1 genes of A. rhizogenes WT strain 15834 into F. tataricum plants was confirmed by PC...

hairy root systems are formed by transforming plant tissues with the “natural genetic engineer” agrobacterium rhizogenes. in most plants such as cichorium intybus l., hairy root cultures have proven to be an efficient system for secondary metabolites production. the effect of zinc (znso4), a heavy metal, was investigated at different concentrations (0, 1, 5 and 10 mm) on some secondary metaboli...

Betalains biosynthesis and antiradical scavenging activity were investigated during cultivation of four hairy root cultures of Beta vulgaris, obtained from different cultivars (Bordo, Egyptian, Detroit 2 and Detroit Dark Red). The best producer of betalains was a hairy root culture from Beta vulgaris cv. Detroit Dark Red (13.27 mg/g dry weight total pigment production). The ethanol extract, der...

Hairy root culture is an effective method to produce secondary metabolites. In fact, hairy roots are genetically and biologically stable and also they are able to produce metabolites without hormones. Chicory (Cichorium intybus L.) is one of the most important medicinal plants with valuable medicinal compounds. In this research, hairy roots were induced by different Agrobacterium rhizogenes str...

background: trigonella foenum-graecum l. commonly known as fenugreek is a rich source of important medicinal metabolite, i.e. trigonelline. objective: in this study, hairy roots culture as a novel method for trigonelline production was evaluated. methods: for optimizing the hairy roots culture of trigonella foenum-graecum, three strains of agrobacterium rhyzogenes (atcc15834, msu440 and k599) v...

Biotic elicitors can be used to stimulate the production of secondary metabolites in plants. However, limited information is available on the effects of biotic elicitors from endophytic fungi on their host plant. Trichoderma atroviride D16 is an endophytic fungus isolated from the root of Salvia miltiorrhiza and previously reported to produce tanshinone I (T-I) and tanshinone IIA (T-IIA). Here,...