Leptohyphes cornutus Allen from Brazil (Santa Catarina) and Misiones, Argentina is re-studied from new material and newly diagnosed as follows: tubercles on head (two pairs), pronotum (two pairs, anterior pair very small), and mesonotum (two pairs), abdominal terga without paired tubercles, nor remnants of them; fore femur length/maximum width, 1.4-1.8; tarsal claws denticulation 4-11+1; hind w...

We have cloned two restriction enzyme fragments from the Hela cell line S3 which hybridize with the genomes of HPV-6 and 16 under conditions of reduced stringency (Tm-43°C). One of the fragments, a 5.7-kb Bam HI fragment, was found to be present within the other, a 15.8-kb Eco RI fragment. Restriction enzyme (RE) mapping of this 15.8-kb fragment (see Figure) revealed that the sequence of RE sit...

A human Hind III 1.9 kb repeated DNA fragment was isolated and cloned in pBR322. A cloned member that hybridized predominantly to the 1.9 kb Hind III band in a digest of whole human DNA was chosen for sequencing. It is an 1894 bp fragment that shows no significant internal repeats. Few pCG residues are observed in the sequence and there are numerous stop codons. Detailed sequence comparisons co...

Mitochondrial DNA from rat liver contains six sites for cleavage by the restriction endonucleases Hind III and EcoRI. A large stretch of DNA, comprising about 40% of the mitochondrial genome is not cleaved by either of the enzymes; eight cleavage sites are located on a DNA stretch of 35% of the genome length suggestive of an unequal distribution of the A - T baspairs over the molecule. The numb...

Casein is a family of milk proteins that exists in several molecular forms and is the main protein present inthe bovine milk. The B variant of bovine k-casein is reported to be favorable for quality and quantity of cheese derived from milk and considered to be included in breeding strategies of dairy cattle. Genotypes of72 Russian Black Pied and 80 Red Pied cows were determined for ...

Mitochondrial DNA of the ascomycete fungus Aspergillus nidulans, a circular molecule of 31 500 base pairs, is cleaved by restriction endonucleases Eco R I, Hind II, Hind III and Bgl II into 3, 7, 9 and 5 fragments, respectively. The relative positions of the cleavage sites could be mapped by analysis of fragments obtained by double enzyme digestions of whole DNA and by complete and partial redi...

A rapid method of ultracentrifugation pelleting of avian adenovirus (AAV) from small volume of chloroform treated infected cell culture fluid or allantoic fluid was adapted for isolation of adenoviral DNA. The viral DNA extracted from semipurified viruses was found to be intact on agarose gel and pure enough (A260/280 = 1.85-1.92) for restriction enzyme analysis. Restriction endonuclease analys...