in this study which has been carried out from 1996 until 1997 soluble latex agglutination antigen was prepared from L, donovani,sudan(s-1) by culturing in special media,harvesting,and later solubilizing by freezing and thawing.the antigen were futher prepared by high speed centrifugation.after measurement of the protein (lowry method) concentration of the antigen was set at 2.2 mg/ml,the antige...

Spermatozoa generate reactive oxygen species (ROS) in physiological amounts which play a role in sperm functions during sperm capacitation acrosome reaction (AR) and oocyte fusion. In addition damaged sperm are likely to be the source of ROS. The most important ROS produced by human sperm are hydrogen peroxide superoxide anion and hydroxyl radicals. Besides human seminal plasma and sperm posses...

In previous studies, mebudipine, a dihydropyridine calcium channel blocker, showed a considerable potential to be used in cardiovascular diseases. The aim of the current study was to develop a valid method using reversed-phase high performance liquid chromatography coupled with electrospray ionization mass spectrometry to assay mebudipine in the human plasma. Separation was achieved on a Zorbax...

Abstract Background Despite improvement in safety of plasma transfusion some virus transmission still remains a problem. So as World Health Organization (WHO) recommends, many countries developed Pathogen Reduction Technologies (PRT) to inactivate pathogens, in plasma components. The Methylene Blue (MB) based methods is one of the most universal one. The purpose of this research was, produce...

there are many evidences that human serum paraoxonase activity modifies plasma lipid profile and paraoxonase has an antiatherogenic property. non-selective beta-blockers affect plasma lipid profile too, but they have atherogenic property when patients take these drugs in long term. in this study the effect of propranolol, a non-selective beta-blocker, on paraoxonase activity was investigated. l...

background: in this study we have prepared the iga solution from normal human plasma using plasma fractionation and ion exchange chromatography. materials and methods: using fractionation of plasma with cold ethanol (starting with 8% ethanol), fraction iii was prepared as a suitable source for iga preparation. then it was treated with caprylic acid for separation of impurities. for enrichment o...

spermatozoa generate reactive oxygen species (ros) in physiological amounts which play a role in sperm functions during sperm capacitation acrosome reaction (ar) and oocyte fusion. in addition damaged sperm are likely to be the source of ros. the most important ros produced by human sperm are hydrogen peroxide superoxide anion and hydroxyl radicals. besides human seminal plasma and sperm posses...

background and objectives: leptospirosis is an important zoonotic disease caused by leptospira interrogans . leptospirosis leads to economical losses in dairy farm industry. the objective of this study was to evaluate the pathogenic serovars of leptospira interrogans in dairy cattle herds of shahrekord by pcr. materials and methods: two hundred samples (100 urine and 100 blood) were collected f...