β-diketone-cobalt complexes, a family of newly synthesized non-platinum metal compounds, exhibit potential antitumor activity; however, the antitumor mechanism is unclear. The current study investigated the mechanism by which β-diketone-cobalt complexes inhibit rat C6 glioma cell proliferation. It was found that β-diketone-cobalt complexes suppress rat C6 glioma cell viability in a dose-depende...

A direct competitive chemiluminescent enzyme-linked immunosorbent assay (CL-ELISA) was developed to determine okadaic acid (OA). Concentrations of the capture monoclonal anti-OA antibodies, conjugate of OA-HRP and a composition of blocking buffers were varied to optimize the assay condition. The values of IC10, IC50 and working range (IC20-IC80) for CL-ELISA were 0.01, 0.07, and 0.03-0.2 ng/mL,...

A simple and reliable enzymatic system for organophosporus pesticide detection was successfully developed, by exploiting the synergy between the magnetic beads collection capacity and the outstanding electrochemistry property of boron-doped diamond electrodes. The determination of an organophosphate pesticide, chlorpyrifos (CPF), was performed based on the inhibition system of the enzyme acetyl...

background: current anti-cancer drug therapy results in systemic side effects due to non-specific uptake by normal healthy noncancerous tissues. to alleviate this difficulty, many attempts have been devoted to the development of new delivery systems such as polymeric nanoparticles (nps). in this study, we prepared icd-85 nps based on sodium alginate and analyzed the cytotoxic activity of icd-85...

A bio-barcode immunoassay based on droplet digital polymerase chain reaction (ddPCR) was developed to simultaneously quantify triazophos, parathion, and chlorpyrifos in apple, cucumber, cabbage, pear. Three gold nanoparticle (AuNP) probes magnetic (MNP) were prepared, binding through their antibodies with the three pesticides same tube. groups of primers, probes, templates, designed ensure spec...

Abstract We present a statistical analysis of the He ii 4686 emission line in spectra black hole and Wolf-Rayet (WR) star high-mass X-ray binary IC10 X-1. This is visibly skewed, third moment (skewness) varies with binary’s orbital phase. describe new method extracting such weak/faint features lying barely above noisy continuum. Using moments these features, we have been able to decompose skewe...