The objective of this work was to characterise an optrA-carrying Enterococcus faecalis ST16 isolate recovered from a river water sample in Switzerland. Linezolid-resistant E. F102 surface Switzerland and subjected comprehensive genotypic characterisation analysis the genetic environment oxazolidinone/phenicol resistance gene optrA. Whole-genome sequencing (WGS) performed detect linezolid mechan...

ABSTRACT A total of 78 isolates of Puccinia triticina from durum wheat from Argentina, Chile, Ethiopia, France, Mexico, Spain and the United States and 10 representative isolates of P. triticina from common wheat from the United States were tested for virulence phenotypes on seedling plants of 35 near-isogenic lines of Thatcher wheat. Isolates with virulence on lines with leaf rust resistance g...

Metarhizium anisopliae is a well-studied entomopathogenic fungus that widely used in biological control programs. The presence of polymycoviruses this common, but their effects on fungal development and stress tolerance are not well understood. In study, we report the discovery novel double-stranded RNA virus, named polymycovirus 1 (MaPmV1), which comprises four dsRNAs ranging from 2.4 to 1.4 k...

A total DNA clone bank of a strain of Xanthomonas campestris pv. malvacearum (Xcm) was constructed in the cosmid vector pSa747 and transfected into Escherichia coli. The Xcm strain carries at least nine identifiable avirulence (A) genes. Clones in E. coli were mated individually into a recombination-proficient Xcm isolate carrying no known A genes. Screening was for incompatibility on congenic ...

Puccinia striiformis f.sp. tritici (Pst) is the causal agent of stripe (yellow) rust on wheat. It seriously threatens wheat production worldwide. The obligate biotrophic fungus is highly capable of producing new virulent races that can overcome resistance. Studying the inheritance of Pst virulence using the classical genetic approach was not possible until the recent discovery of its sexual sta...

as the host plants based on their physical and chemical characteristics can affect virulence of microbial control agents on the target pest and its natural enemies, therefore in the current study, the virulence of beauveria bassiana tv isolate at concentration 107 conidia/ml was evaluated against t. urticae female adults, on three different host plants; tomato, cucumber and strawberry. spray to...

Sequence tagged site (STS) markers for eight resistance genes against Puccinia recondita f. sp. tritici were used to screen a set of near-isogenic lines of wheat cv. Thatcher containing in total 40 different Lr genes and their alleles. Polymerase chain reaction (PCR) analysis was carried out by using STS, SCAR and CAPS primers specific for the leaf rust resistance genes Lr1, Lr9, Lr10, Lr19, Lr...

The interaction between Arabidopsis thaliana and the bacterium Pseudomonas syringae is being developed as a model experimental system for plant pathology research. Race-specific ("gene-for-gene") resistance has been demonstrated for this interaction, and pathogen genes that determine avirulence have been isolated and characterized. Because certain lines of both Arabidopsis and soybean are resis...