Macrophage multinucleation (MM) is essential for various biological processes such as osteoclast-mediated bone resorption and multinucleated giant cell-associated inflammatory reactions. Here we study the molecular pathways underlying multinucleation in the rat through an integrative approach combining MS-based quantitative phosphoproteomics (LC-MS/MS) and transcriptome (high-throughput RNA-seq...

prosite database contains a set of entries corresponding to protein families, which are used to identify the family of a protein from its sequence. although patterns and profiles are developed to be very selective, each may have false positive or negative hits. considering false positives as items that reduce the selectiveness of a pattern, then, the more selective pattern we have, a more accur...

BACKGROUND GC-binding factor 2 (GCF2) is a transcriptional regulator that represses transcriptional activity of the epidermal growth factor receptor (EGFR) by binding to a specific GC-rich sequence in the EGFR gene promoter. In addition to this function, GCF2 has also been identified as a tumor-associated antigen and regarded as a potentially valuable serum biomarker for early human hepatocellu...

recombinant protein purification is a kind of sensitive and expensive method in genetics engineering. genetic manipulation leads to the expression of various proteins; it should be isolated with high purity finally. differed methods for protein purification are categorized, based on cast, quality, easy work and side effect of protein. in this article, we are investigating his his-tag protein pu...

background: in vitro simulation of developmental processes is an invaluable tool to shed light on the intrinsic mechanism of developmental biosystems such as central nervous system in mammals. chick somites have been used to simulate the neural differentiation of human neural progenitor cells. in the present study, we aimed to indicate whether somites have the ability to express required neural...

a sweet water-soluble protein that reminds stable over wide ranges of temperature and ph, brazzein has various applications. its tastes like cane sugar but have no calories. however, the extraction of brazzein from its natural source is expensive and not applicable. in this study we used recombinant dna technology to provide an alternative option for cheaper mass production of brazzein. a brazz...

we present the first reported case of antiphospholipid syndrome with stroke in an iranian boy (7-month-old) who had two ischemic strokes within a period of 2 months. serum anticardiolipid antibody was positive and the patient had low levels of protein s and c. this case emphasizes the importance of antiphospholipid antibody in children with unexplained ischemic stroke.