The protozoan parasite Toxoplasma gondii causes severe opportunistic infections. Here, we report an unexpected diagnosis of cerebral toxoplasmosis. T. gondii was diagnosed by 16S and D2 large-subunit (LSU) ribosomal DNA (rDNA) sequencing of a cerebral biopsy specimen and confirmed by T. gondii-specific PCR and immunohistochemistry. The patient was later diagnosed with HIV/AIDS.

An insertion of one or two amino acids at the monomer-monomer junctions of polyubiquitin is a distinct and highly conserved molecular character that is shared by two very diverse clades of microeukaryotes, the Cercozoa and the Foraminifera. It has been suggested that an insertion consisting of one amino acid, like that found in foraminiferans and some cercozoans, represents an ancestral state, ...

A large number of group I introns were discovered in coding regions of small and large subunits of nuclear ribosomal RNA genes (SSU rDNA and LSU rDNA) in ascomycetous fungi of the genus CORDYCEPS: From 28 representatives of the genus, we identified in total 69 group I introns which were inserted at any of four specific sites in SSU rDNA and four specific sites in LSU rDNA. These group I introns...

An unusual population of Meloidogyne hapla, earlier thought to be an undescribed species, was found causing large galls, without adventitious roots, and substantial damage to coffee in Maui, Hawaii. Only in Brazil had similar damage to coffee been reported by this species. Unlike M. exigua from South and Central America, this population reproduced well on coffee cv. Mokka and M. incognita-susce...

Parsimony analysis of sequences of the internal transcribed spacer region of the nuclear rDNA and partial sequences of the large subunit (LSU) place four anamorphic Chalara species as a monophyletic grouping within the teleomorph genus Ceratocystis. Chalara ovoidea, Ch. thielavioides, Ch. populi, and Ch. elegans (synanamorph: Thielaviopsis basicola) form aleurioconidia typical of the anamorph g...

A macrofungi species, Cortinarius bulliardii is collected from Tokat city province during field trips in 2019. It has a smooth, finely light fibrous, brownish-red pileus, whitish to lilac-brownish flesh, broadly ellipsoidal spores, brown when young, then dark lamellae with age. In addition morphological analysis, molecular sequence data based on the ITS and LSU rDNA gene regions indicated that ...

Abstract Taxonomy of Pseudohydnum gelatinosum and its sister taxa is revised via morphological data a four-gene dataset (ITS, nc LSU rDNA, TEF1 , RPB1 ). Identity P. Tremellodon pusillus re-established based on newly collected sequenced material from their type localities. alienum Europe; umbrosum temperate East Asia; cystidiatum meridianum placibile Vietnam; omnipavum North America are describ...

Powdery mildews are a group of economically and ecologically important plant pathogens. In the past 25 years use ribosomal DNA (rDNA) in powdery has led to major taxonomic revisions. However, broad scale rDNA also revealed multiple species complexes that cannot be differentiated based on ITS + LSU data alone. Currently, there only two mildew studies took multi-locus approach resolve complex. pr...