the aim of this study was to investigate the frequency, location and type of rpob gene mutations in mycobacterium tuberculosis (mtb) collected from patients in the southern endemic region of iran. drug susceptibility testing was determined by using the bactec system and the center for diseases control’s (cdc) standard conventional proportional method. in 29 rifampicin-resistant mtb (85%) isolat...

Three tuberculous, twenty-one non-tuberculous mycobacterial (NTM) reference strains and seventy two isolates classified by biochemical tests were shown to produce specific sets of DNA fragments in a polymerase chain reaction with single universal primer (UP-PCR). A rather wide limit of tolerance for variations in procedure of PCR mixture preparation and thermocycling parameters was found. There...

conclusions the data indicate that esat6-cfp10 could support the survival of m. tuberculosis in the host through altering the host immune response. it also indicates that the host may gain some level of protection from a second exposure to m. tuberculosis, as evidenced by increased proliferation of nr8383-ec. background mycobacterium tuberculosis is the major pathogen of tuberculosis, which aff...

Background & Objectives: Tuberculosis is one of the greatest health problems in Iran. The distribution of the disease is not equal in all parts of the country. The aim of this study was to evaluate the frequency of positive results for Mycobacterium tuberculosis in samples referred to an academic hospital in an 8 year period. Materials and Methods: The samples from different wards of ...

objective: the rifampicin resistance and susceptibility of mycobacterium tuberculosis are caused by mutations in the 81-base pair region of the rpob gene encoding the b-subunit of rna polymerase. methods: isoniazid resistance of m. tuberculosis is related to mutations in inha , oxyr and ahpc genes which 30 to 90 percent of isoniazid resistance is occurred in 3015 codons of katg gene. the rpob a...

Objective(s): The periodic binding of protein expressed by Mycobacterium tuberculosis H37Rv with the host cell receptor molecules i.e. fibronectin (Fn) is gaining significance because of its adhesive properties.  The genome sequencing of M. tuberculosis H37Rv revealed that the proline-glutamic (PE) proteins contain polymorphic GC-rich repetitive sequences (PGRS) which have clinical importance i...

Introduction: In HIV-associated tuberculosis cases, identifying nontuberculous Mycobacteria (NTM) from clinical samples has become essential regarding patients’ treatment and prognosis. This study aims to determine the prevalence of different Mycobacteria species from immunocompromised and immunocompetent patients with suspected tuberculosis attending a tertiary care hospital in Kolkata, India....

conclusions in this experimental study, for the first time in iran the expression and purification of this recombinant protein was done successfully. this recombinant protein could be used as a vaccine candidate and diagnostic purpose in subsequent investigations. results the highest expression of the cytochrome p450 cyp141 protein was obtained by the addition of 1 mm of isopropyl β-d-1-thiogal...

We investigated the use of DNA amplification by polymerase chain reaction (peR) for detection of Mycobacterium tuberculosis in 300 patients who were suspected of having pulmonary tuberculosis and compared the results with culture results which were performed in parallel with PCR. Two-thirds of each sample was processed for smear and culture by standard methods and one-third was prepared fo...