A new method for the preparation of the glycoforms of bovine a1-acid glycoprotein (AGP) is described relying on affinity-reversed micellar extraction and separation (ARMES). This method has proven effective in separating structurally similar glycoproteins and separating glycoproteins from nonglycosylated proteins from natural sources. In this method, individual glycoforms complex with the lecti...

This paper presents new methods of determination of sulfonamide residues in food products of animal origin, based on liquid chromatography with a micellar mobile phase. The methods employ a technique of direct injection of the sample and preliminary isolation of the analyte by extraction in the liquid-solid and liquid-liquid system. The methods have been characterized by providing the parameter...

This paper describes a micellar liquid chromatographic method used to analyze tamoxifen (TAMO) in pharmaceutical formulations, while focusing in its interesting features. Solid samples were solved in a micellar solution, irradiated at 254 nm, filtered and injected. Extraction steps were avoided and thus expediting the procedure. Tamoxifen was resolved in <5 min, using a mobile phase containing ...

A new method for the preparation of the glycoforms of bovine alpha(1)-acid glycoprotein (AGP) is described relying on affinity-reversed micellar extraction and separation (ARMES). This method has proven effective in separating structurally similar glycoproteins and separating glycoproteins from nonglycosylated proteins from natural sources. In this method, individual glycoforms complex with the...

A micellar liquid chromatographic procedure is described for simultaneous determination of sugars previously derivatized with 4-aminobenzoic acid ethyl ester (4-ABEE). The optimized conditions, determined by use of the super-modified simplex method, were temperature 30°C, mobile phase containing 17.3 mM sodium dodecyl sulfate, 0.01 M phosphate buffer, pH 6.69, and 7.73% (v/v) ethanol, and UV de...

Reverse micellar systems are nanofluids with unique properties that make them attractive in high selectivity separation processes, especially for biological compounds. Understanding the phase behavior and thermodynamic properties of these nanosystems is the first step in process design. Separation of components by these nanosystems is performed upon contact of aqueous and reverse micellar phase...