AIM This cross-sectional study aims to assess the prevalence of Cryptosporidium in hemodialysis patients compared with healthy individuals in central Iran from August 2014 to January 2015. BACKGROUND Cryptosporidiosis is a major cause of acute and persistent diarrhea with significant morbidity and mortality in immunocompromised patients such as those undergoing renal dialysis. METHODS Three...

Background The sensitivity of smear microscopy for diagnosis of tuberculosis might be improved through treatment of sputum with sodium hypochlorite and application of fluorescent microscopy. This study aimed to determine the agreement between direct Fluorescent Microscopy and Ziehl-Neelsen concentration technique by their ability of detecting acid fast bacilli in resource poor settings. Metho...

Some techniques available at our laboratory were tested for their ability to aid in the morphological diagnosis of hydatid elements (Echinococcus granulosus ["Taenia echinococcus"]) isolated from cysts in humans and sheep. Unstained, methanol-fixed hooklets were fluorescent, most starkly so under violet light (excitation filter wavelength, 405 nm; long-pass filter wavelength, 495 nm). Auramine-...

Cryptosporidium sp. is a zoonotic intestinal protozoa in orangutans which transmitted through contaminated water (waterborne disease). This study aims to determine infects the sumatran orangutan (Pongo abelii) and bornean pygmaeus) North Sumatera Wildlife Park. The samples used were 3 fecal from different locations, two Medan Park one Pematang Siantar Animal Samples examined using floatation me...

AIMS To evaluate a safe sputum processing method for detection of tuberculosis in developing countries. METHODS A sample processing method was developed in which acid fast bacilli were killed with 1% sodium hypochlorite and concentrated by flotation on a layer of xylene before staining by the Ziehl Neelsen or auramine O methods. RESULTS Best results were obtained by auramine O staining afte...

Cyclospora cayetanensis is a cause of prolonged diarrhoea, mainly in travellers. Laboratory diagnosis may be achieved by a number of methods such as the staining of faecal smears by the modified Ziehl-Neelsen (ZN) technique. Safer methods using this technique have been described for the staining of acid fast bacilli and cryptosporidia by replacing the phenol content of the carbol fuschin stain ...

The stool samples from 363 diarrhoeic and non- diarrhoeic children and adults from several locations in two towns of west Azerbaijan area of Iran examined for Cryptosporidium infection by modified Ziehl- Neelsen technique revealed the presence of infection in 7.66% of diarrhoeic patients and in none of the non- diarrhoeic individuals. The percentage of detection was higher among females ( ...

the stool samples from 363 diarrhoeic and non- diarrhoeic children and adults from several locations in two towns of west azerbaijan area of iran examined for cryptosporidium infection by modified ziehl- neelsen technique revealed the presence of infection in 7.66% of diarrhoeic patients and in none of the non- diarrhoeic individuals. the percentage of detection was higher among females ( 11.21...

This is a prospective, descriptive and analytic study conducted from July 2011 to September 2011 at the Children National Hospital Albert Royer of Dakar and at the Vélingara Health District. It was focused on children under 15 without reference to HIV status. For each child, a sample of stool was examined by the Ziehl-Neelsen modified staining and by ELISA using the “Cryptosporidium Antigen Det...

to investigate the prevalence of cryptosporidium infection in horses, a total of 200 fecal specimens were collected randomly from five farms in tehran. samples were taken during a year, 50 specimens each season. of these specimens, 57 were obtained from caspian pony. the presence of oocysts in the samples was confirmed by modified ziehl-neelsen staining of direct smears of the fecal material fr...