DNA size markers (ladder) are essential tools in molecular biology, genetics and biotechnology. In this study, a simple and cost-effective method for laboratory production of DNA ladders is introduced. For this purpose, different sizes of 100 to 2000 bp DNA segments were designed using PCR technique. For producing 14 different gene fragments as DNA molecular weight markers, recombinant plasmid ...

Die-back disease caused by Paecilomyces variotti is one of the most destructive diseases of pistachio in Iran. In this study, genetic diversity of Paecilomyces variotti isolates was investigated with SSR marker, using six primers pairs. Sampling was done from pistachio gardens in different geographical zones of Kerman province during summer 2013. Various fungal isolates were isolated from infec...

background and objectives : dna molecular weight marker is widely used in molecular biology experiments incurring considerable costs on low-budget settings. materials and methods : here a pcr-supported procedure is described that uses 10 primer pairs targeting chromosomal dna from the harmless vaccinal bacillus anthracis sterne 34f2 strain as template. a single pcr protocol is used to reproduce...

The aim of this study was to evaluate the utility of COI, of the mitochondrial DNA, for the taxonomic determination of soft-bottom benthic polychaetes in the Chiloé Inner Sea as a particularly important zone for polychaete fauna of the southeast Pacific. The results show high genetic differentiation between morphological species of polychaetes (posterior probabilities upper 0.9), and Onuphis ps...

For almost twenty years now, huge advances in the field of molecular biology have made it possible to identify molecules or molecular markers that represent the signature of a serious pathology, I mean cancer in particular. Thus, rapid detection enables to envisage first-line treatments such as surgical ablation, for example, which would avoid treatment that is heavier and more invasive for the...

Background & Aims: SLC26A4 gene mutations are the second identifiable genetic cause of autosomal recessive nonsyndromic hearing loss (ARNSHL) after GJB2 mutations and are currently investigated in molecular diagnosis.In databases, several potential STR markers related to this region have been introduced. In this investigation, the characteristics and informativeness of D7S2425 CA repeat STR mar...