The therapeutic use of antibodies is restricted by the limited access of antibodies to intracellular compartments. To overcome this limitation, we developed a cell-penetrating monoclonal antibody, mAb 3E10, as an intracellular delivery vehicle for the intracellular and intranuclear delivery of antibodies constructed as bispecific single-chain Fv fragments. Because MDM2 is an important target in...

The therapeutic use of antibodies is restricted by the limited access of antibodies to intracellular compartments. To overcome this limitation, we developed a cell-penetrating monoclonal antibody, mAb 3E10, as an intracellular delivery vehicle for the intracellular and intranuclear delivery of antibodies constructed as bispecific single-chain Fv fragments. Because MDM2 is an important target in...

Monoclonal Antibodies, also known as mAbs or mOAbs, are artificial antibodies produced in laboratories. These proteins assist the immune system recognizing, tagging, and destroying cells a number of different ways. By tagging antigens foreign when body is unable to recognize these cells, they activate destroy cells. largely preferred over polyclonal due their specificity targeted treatment. Thi...

despite the major advances in conventional forms of treatment (i.e. surgical techniques, radiotherapy and chemotherapy) and improved survival rates, cancer is still the second leading cause of death in developing countries. one major limitation of cytotoxic drugs and radiation in the treatment of cancer patients is their inability to discriminate between malignant and normal tissues. this in tu...

cd34 is a type i membrane protein with a molecular mass of approximately 110 kda. this antigen is associated with human hematopoietic progenitor cells and is a differentiation stage-specific leukocyte antigen. in this study we have generated and characterized monoclonal antibodies (mabs) directed against a cd34 marker. mice were immunized with two keyhole lympet hemocyanin (klh)-conjugated cd34...

Hybridoma cells which synthesize monoclonal antibodies (mAb) that block ricin toxicity were 50-300-fold resistant to ricin compared with other hybridomas. Two of the mAb blocked two isozymes of ricin, D and E, to different and opposite extents, and the hybridoma cell resistance to the two forms of ricin closely corresponded with the mAb reactivity. The hybridoma cell resistance to ricin was the...

Foot-and-mouth disease (FMD) is a major viral in farm animals. In the present study, seven monoclonal antibodies (mAbs) were produced against FMD virus (FMDV)-encoded RNA-dependent RNA polymerase (3D protein) and characterized. Screening of mAb reactivity three overlapping fragments 3D protein expressed Escherichia coli revealed that binding sites all mAbs confined to N-terminal one-third prote...

two igm monoclonal antibodies (mab) with strong reactivity for granulocytes and to a lesser extent for lurkat cell lines were established by immunizing balbi c mice with a histiocytic cell line (uy37). these two mabs (designated as 6c9 and 4c4) reacted with blast cells of t-acute lymphoblastic leukemia (t-all) and acute myelogenous leukemia (aml) patients as well as leukemic cells from patients...

Background: The therapy of leishmania infection is difficult and each year 1.5 million new cases of cutaneous leishmaniasis and 500,000 new cases of visceral leishmaniasis are estimated, therefore, there is a need for an effective vaccine. Monoclonal antibody (mAb) is one of the suitable methods for isolation and purification of leishmania antigens. In this report, we produced several mAb aga...