Respirasomes are macromolecular assemblies of the respiratory chain complexes I, III and IV in the inner mitochondrial membrane. We determined the structure of supercomplex I1III2IV1 from bovine heart mitochondria by cryo-EM at 9 Å resolution. Most protein-protein contacts between complex I, III and IV in the membrane are mediated by supernumerary subunits. Of the two Rieske iron-sulfur cluster...

Using laser fluctuation spectroscopy, a technique that measures particle size change in solution, the kinetics of fibrin clot formation from fibrinogen can be studied. With this technique the effect of calcium on the three distinguishable phases of clot formation, (1) proteolysis of fibrinogen, (2) fibrinogen-fibrin monomer complex formation, and (3) fibrin monomer polymerization, were investig...

The determinants of monomer activities of lipids dissolved in micellar bile salt solutions have been studied using polyethylene discs as the organic phase of a partitioning system. The studies show that fatty acids and alcohols interact with micelles as a partitioning system so that the monomer activity is determined by micelle volume and the lipid's partition coefficient as well as mass of lip...

In the present study, silver nanoparticles were synthesized by decomposition of AgNO3 via gelcasting. Methacrylamide was used as a low toxic monomer for gel formation. Effect of monomer content (MAM), cross-linker to monomer ratio (MBAM/MAM), silver salt to monomer ratio (AgNO3/MAM), duration of calcination, and temperature of calcination were investigated to optimize the size of nanoparticles....

Using urea-solubilized human fibrin monomer as an immunogen, we raised in mice a battery of monoclonal antibodies that reacted with the immunogen but not with urea-treated or native fibrinogen. Although they all failed to react with acid-solubilized fibrin monomer (acid-FM) alone, an antibody designated as IF-43 was found to recognize acid-FM, which was bound with fibrinogen or its derivatives ...